Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols[S]

Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols[S]

Iso-octyl chain-hydroxylated oxysterols were determined in attomoles per 10,000 cells concentrations in 10,000–80,000 cultured pancreatic adenocarcinoma cells, using a sensitive, highly automated nano-LC-ESI-MS-based method. Identified oxysterols included 24S hydroxycholesterol (24S-OHC), 25 hydroxy...

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Bibliographic Details
Main Authors: Hanne Roberg-Larsen, Kaja Lund, Tore Vehus, Nina Solberg, Caroline Vesterdal, Dorna Misaghian, Petter Angell Olsen, Stefan Krauss, Steven Ray Wilson, Elsa Lundanes
Format: Article
Language:English
Published: Elsevier 2014-07-01
Series:Journal of Lipid Research
Subjects:
24S hydroxycholesterol
25 hydroxycholesterol
27 hydroxycholesterol
cholesterol 24-hydroxylase
cholesterol 25-hydroxylase
cholesterol 27-hydroxylase
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520352585

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http://www.sciencedirect.com/science/article/pii/S0022227520352585

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