Highly sensitive electrochemical assay based on strand displacement polymerization-assisted CRISPR/Cas12a collateral cleavage

Highly sensitive electrochemical assay based on strand displacement polymerization-assisted CRISPR/Cas12a collateral cleavage

In this work, a novel strand displacement polymerization strategy is designed with a single hairpin-structured DNA probe acting as both of primer and template. After target miRNA-induced structural transitions, extension and displacement reactions occur at the dimer with recycled target miRNA. The g...

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Bibliographic Details
Main Authors: Yulin Zhu, Xifeng Chen, Jiayue Shi, Haixuan Sun, Hua Chai, Peng Miao
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Electrochemistry Communications
Subjects:
miRNA
CRISPR/Cas12a
Strand displacement amplification
Silver nanoparticles
Hairpin assembly
Online Access:http://www.sciencedirect.com/science/article/pii/S1388248123002047

Internet

http://www.sciencedirect.com/science/article/pii/S1388248123002047

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