Quantifying RNA and DNA in planktonic organisms with SYBR Green II and nucleases. Part B. Quantification in natural samples

Quantifying RNA and DNA in planktonic organisms with SYBR Green II and nucleases. Part B. Quantification in natural samples

Extraction procedures for RNA and DNA in crude extracts of natural microplankton samples are developed. The methodology is compatible with the fluorometric assay of RNA and DNA developed by Berdalet et al. (2005). Mechanical cell disruption using a manual tissue grinder is combined with chemical ext...

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Bibliographic Details
Main Authors: Elisa Berdalet, Cristina Roldán, M. Pilar Olivar
Format: Article
Language:English
Published: Consejo Superior de Investigaciones Científicas 2005-03-01
Series:Scientia Marina
Subjects:
sybr green ii
dnase
rnase
sarcosine
rna/dna ratios
plankton
Online Access:http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/231

Internet

http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/231

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