Efficient Recreation of t(11;22) EWSR1-FLI1+ in Human Stem Cells Using CRISPR/Cas9

Efficient Recreation of t(11;22) EWSR1-FLI1+ in Human Stem Cells Using CRISPR/Cas9

Efficient methodologies for recreating cancer-associated chromosome translocations are in high demand as tools for investigating how such events initiate cancer. The CRISPR/Cas9 system has been used to reconstruct the genetics of these complex rearrangements at native loci while maintaining the arch...

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Bibliographic Details
Main Authors: Raul Torres-Ruiz, Marta Martinez-Lage, Maria C. Martin, Aida Garcia, Clara Bueno, Julio Castaño, Juan C. Ramirez, Pablo Menendez, Juan C. Cigudosa, Sandra Rodriguez-Perales
Format: Article
Language:English
Published: Elsevier 2017-05-01
Series:Stem Cell Reports
Subjects:
CRISPR
cancer translocation
human stem cells
genome engineering
Ewing sarcoma
MSC
iPSC
cancer modeling
Cas9
disease model
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671117301686

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http://www.sciencedirect.com/science/article/pii/S2213671117301686

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