The Spermidine Synthase Gene <i>SPD1</i>: A Novel Auxotrophic Marker for <i>Chlamydomonas reinhardtii</i> Designed by Enhanced CRISPR/Cas9 Gene Editing

The Spermidine Synthase Gene <i>SPD1</i>: A Novel Auxotrophic Marker for <i>Chlamydomonas reinhardtii</i> Designed by Enhanced CRISPR/Cas9 Gene Editing

Biotechnological application of the green microalga <i>Chlamydomonas reinhardtii</i> hinges on the availability of selectable markers for effective expression of multiple transgenes. However, biological safety concerns limit the establishment of new antibiotic resistance genes and until...

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Bibliographic Details
Main Authors: Robert A. Freudenberg, Luisa Wittemeier, Alexander Einhaus, Thomas Baier, Olaf Kruse
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Cells
Subjects:
microalgae
CRISPR/Cas9
gene editing
spermidine
<i>SPD1</i>
auxotrophy
Online Access:https://www.mdpi.com/2073-4409/11/5/837

Internet

https://www.mdpi.com/2073-4409/11/5/837

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