Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In Vitro
Cyclodextrins (CDs) are promising drug carriers that are used in medicine. We chose CDs with different substituents (polar/apolar, charged/neutral) to obtain polymers (CDpols) with different properties. CDpols are urethanes with average Mw of ~120 kDa; they form nanoparticles 100–150 nm in diameter...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/21/4476 |
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| author | Anna A. Skuredina Anastasia S. Tychinina Irina M. Le-Deygen Sergey A. Golyshev Tatiana Yu. Kopnova Nikolay T. Le Natalya G. Belogurova Elena V. Kudryashova |
| author_facet | Anna A. Skuredina Anastasia S. Tychinina Irina M. Le-Deygen Sergey A. Golyshev Tatiana Yu. Kopnova Nikolay T. Le Natalya G. Belogurova Elena V. Kudryashova |
| author_sort | Anna A. Skuredina |
| collection | DOAJ |
| description | Cyclodextrins (CDs) are promising drug carriers that are used in medicine. We chose CDs with different substituents (polar/apolar, charged/neutral) to obtain polymers (CDpols) with different properties. CDpols are urethanes with average Mw of ~120 kDa; they form nanoparticles 100–150 nm in diameter with variable ζ-potential. We studied the interaction of CD and CDpols with model (liposomal) and bacterial membranes. Both types of CD carriers cause an increase in the liposomal membrane permeability, and for polymers, this effect was almost two times stronger. The formation of CD/CDpols complexes with levofloxacin (LV) enhances LV’s antibacterial action 2-fold in vitro on five bacterial strains. The most pronounced effect was determined for LV-CD complexes. LV-CDs and LV-CDpols adsorb on bacteria, and cell morphology influences this process dramatically. According to TEM studies, the rough surface and proteinaceous fimbria of Gram-negative <i>E. coli</i> facilitate the adsorption of CD particles, whereas the smooth surface of Gram-positive bacteria impedes it. In comparison with LV-CDs, LV-CDpols are adsorbed 15% more effectively by <i>E. coli</i>, 2.3-fold better by lactobacilli and 5-fold better in the case of <i>B. subtilis</i>. CDs and CDpols are not toxic for bacterial cells, but may cause mild defects that, in addition to LV-CD carrier adsorption, improve LV’s antibacterial properties. |
| first_indexed | 2024-03-09T18:44:09Z |
| format | Article |
| id | doaj.art-783eec2340f54993ad11f54fa6c7dac2 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T18:44:09Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-783eec2340f54993ad11f54fa6c7dac22023-11-24T06:26:52ZengMDPI AGPolymers2073-43602022-10-011421447610.3390/polym14214476Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In VitroAnna A. Skuredina0Anastasia S. Tychinina1Irina M. Le-Deygen2Sergey A. Golyshev3Tatiana Yu. Kopnova4Nikolay T. Le5Natalya G. Belogurova6Elena V. Kudryashova7Chemistry Department, Lomonosov MSU, 119991 Moscow, RussiaChemistry Department, Lomonosov MSU, 119991 Moscow, RussiaChemistry Department, Lomonosov MSU, 119991 Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov MSU, 119991 Moscow, RussiaChemistry Department, Lomonosov MSU, 119991 Moscow, RussiaFaculty of Physics, Lomonosov MSU, 119991 Moscow, RussiaChemistry Department, Lomonosov MSU, 119991 Moscow, RussiaChemistry Department, Lomonosov MSU, 119991 Moscow, RussiaCyclodextrins (CDs) are promising drug carriers that are used in medicine. We chose CDs with different substituents (polar/apolar, charged/neutral) to obtain polymers (CDpols) with different properties. CDpols are urethanes with average Mw of ~120 kDa; they form nanoparticles 100–150 nm in diameter with variable ζ-potential. We studied the interaction of CD and CDpols with model (liposomal) and bacterial membranes. Both types of CD carriers cause an increase in the liposomal membrane permeability, and for polymers, this effect was almost two times stronger. The formation of CD/CDpols complexes with levofloxacin (LV) enhances LV’s antibacterial action 2-fold in vitro on five bacterial strains. The most pronounced effect was determined for LV-CD complexes. LV-CDs and LV-CDpols adsorb on bacteria, and cell morphology influences this process dramatically. According to TEM studies, the rough surface and proteinaceous fimbria of Gram-negative <i>E. coli</i> facilitate the adsorption of CD particles, whereas the smooth surface of Gram-positive bacteria impedes it. In comparison with LV-CDs, LV-CDpols are adsorbed 15% more effectively by <i>E. coli</i>, 2.3-fold better by lactobacilli and 5-fold better in the case of <i>B. subtilis</i>. CDs and CDpols are not toxic for bacterial cells, but may cause mild defects that, in addition to LV-CD carrier adsorption, improve LV’s antibacterial properties.https://www.mdpi.com/2073-4360/14/21/4476cyclodextrinlevofloxacinliposomespectroscopyantibacterial activity |
| spellingShingle | Anna A. Skuredina Anastasia S. Tychinina Irina M. Le-Deygen Sergey A. Golyshev Tatiana Yu. Kopnova Nikolay T. Le Natalya G. Belogurova Elena V. Kudryashova Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In Vitro Polymers cyclodextrin levofloxacin liposome spectroscopy antibacterial activity |
| title | Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In Vitro |
| title_full | Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In Vitro |
| title_fullStr | Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In Vitro |
| title_full_unstemmed | Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In Vitro |
| title_short | Cyclodextrins and Their Polymers Affect the Lipid Membrane Permeability and Increase Levofloxacin’s Antibacterial Activity In Vitro |
| title_sort | cyclodextrins and their polymers affect the lipid membrane permeability and increase levofloxacin s antibacterial activity in vitro |
| topic | cyclodextrin levofloxacin liposome spectroscopy antibacterial activity |
| url | https://www.mdpi.com/2073-4360/14/21/4476 |
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