Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies
Research background. Tomato (Solanum lycopersicum L.) fruit is highly consumed worldwide and contains high amounts of carotenoids and tocopherols, two powerful antioxidants. Native tomato genotypes are rarely used in large-scale market but serve as a reservoir to diversify the species gene pool and...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2022-01-01
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| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | https://hrcak.srce.hr/file/398542 |
| _version_ | 1797206727209254912 |
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| author | Darío R. Gómez-Linton Arturo Navarro-Ocaña Silvestre Alavez Ricardo Lobato-Ortiz Angélica Román-Guerrero José Alberto Mendoza-Espinoza Juan Manuel Villa-Hernández Laura J. Pérez-Flores |
| author_facet | Darío R. Gómez-Linton Arturo Navarro-Ocaña Silvestre Alavez Ricardo Lobato-Ortiz Angélica Román-Guerrero José Alberto Mendoza-Espinoza Juan Manuel Villa-Hernández Laura J. Pérez-Flores |
| author_sort | Darío R. Gómez-Linton |
| collection | DOAJ |
| description | Research background. Tomato (Solanum lycopersicum L.) fruit is highly consumed worldwide and contains high amounts of carotenoids and tocopherols, two powerful antioxidants. Native tomato genotypes are rarely used in large-scale market but serve as a reservoir to diversify the species gene pool and can be employed to obtain functional compounds. Extraction methods are currently changing towards cleaner procedures that are more efficient and environmentally friendly, including avoiding toxic or polluting solvents.
Experimental approach. In this study, factorial and fractional factorial designs were used to evaluate the efficiency of digestive enzymes, sonication and green solvents to obtain lipophilic antioxidant extracts from native tomato. To monitor the efficiency of the extraction process, spectrophotometric quantification of total carotenoids and antioxidant activity was carried out, and then individual quantification of carotenoids and tocopherols in the extracts was done by HPLC.
Results and conclusions. Digestive enzymes and sonication increased the carotenoid content and the antioxidant activity of the obtained extracts when applied individually. However, when these treatments were applied together and in combination with isopropyl acetate, a green solvent, the obtained extracts had the highest carotenoid and tocopherol contents as well as the maximal antioxidant activity. A correlation analysis suggested that antioxidant activity resulted from synergistic effects rather than individual compounds. Tomato extracts were obtained through a rapid and environmentally friendly extraction method and their antioxidant activity was enhanced.
Novelty and scientific contribution. Tomato fruits have been the subject of numerous studies; however, functional compound extraction through environmentally friendly methods remains an attractive use of native tomato fruit, enhancing its limited production and harnessing a large amount of tomato product industry. There are few reports where environmentally friendly extraction methods are combined; even rarer are those where green solvents are also used. In this work, the combination of different environmentally friendly extraction methods improved the extraction of carotenoids and tocopherols and allowed to establish a more efficient process. These results could stimulate the use of clean technologies and make the native tomato more attractive for industrial or compound extraction processes. |
| first_indexed | 2024-04-24T09:11:36Z |
| format | Article |
| id | doaj.art-787d60307f0e48bfb8be46bbc280a22f |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:11:36Z |
| publishDate | 2022-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-787d60307f0e48bfb8be46bbc280a22f2024-04-15T17:38:21ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062022-01-0160112113110.17113/ftb.60.01.22.7366Extraction of Lipophilic Antioxidants from Native Tomato Using Green TechnologiesDarío R. Gómez-Linton0Arturo Navarro-Ocaña1Silvestre Alavez2Ricardo Lobato-Ortiz3Angélica Román-Guerrero4José Alberto Mendoza-Espinoza5Juan Manuel Villa-Hernández6Laura J. Pérez-Flores7Biotechnology Ph.D. Program, Metropolitan Autonomous University, Iztapalapa Campus, F.F. C.C. St. Rafael Atlixco Ave. 186, 09310, Mexico City, MexicoNational Autonomous University of Mexico, Outer Circuit, Coyoacan, 04510, Mexico City, MexicoMetropolitan Autonomous University, Lerma Unit, Herons Ave. 10, 52005, Lerma de Villeda, Mexico State, MexicoMetropolitan Autonomous University, Lerma Unit, Herons Ave. 10, 52005, Lerma de Villeda, Mexico State, MexicoMetropolitan Autonomous University, Iztapalapa Campus, F.F. C.C. St. Rafael Atlixco Ave. 186, 09310, Iztapalapa, Mexico City, MexicoAutonomous University of Mexico City, Liberty House Campus, Ermita Iztapalapa Road 4163, 09620, Iztapalapa, Mexico City, MexicoMetropolitan Autonomous University, Iztapalapa Campus, F.F. C.C. St. Rafael Atlixco Ave. 186, 09310, Iztapalapa, Mexico City, MexicoMetropolitan Autonomous University, Iztapalapa Campus, F.F. C.C. St. Rafael Atlixco Ave. 186, 09310, Iztapalapa, Mexico City, MexicoResearch background. Tomato (Solanum lycopersicum L.) fruit is highly consumed worldwide and contains high amounts of carotenoids and tocopherols, two powerful antioxidants. Native tomato genotypes are rarely used in large-scale market but serve as a reservoir to diversify the species gene pool and can be employed to obtain functional compounds. Extraction methods are currently changing towards cleaner procedures that are more efficient and environmentally friendly, including avoiding toxic or polluting solvents. Experimental approach. In this study, factorial and fractional factorial designs were used to evaluate the efficiency of digestive enzymes, sonication and green solvents to obtain lipophilic antioxidant extracts from native tomato. To monitor the efficiency of the extraction process, spectrophotometric quantification of total carotenoids and antioxidant activity was carried out, and then individual quantification of carotenoids and tocopherols in the extracts was done by HPLC. Results and conclusions. Digestive enzymes and sonication increased the carotenoid content and the antioxidant activity of the obtained extracts when applied individually. However, when these treatments were applied together and in combination with isopropyl acetate, a green solvent, the obtained extracts had the highest carotenoid and tocopherol contents as well as the maximal antioxidant activity. A correlation analysis suggested that antioxidant activity resulted from synergistic effects rather than individual compounds. Tomato extracts were obtained through a rapid and environmentally friendly extraction method and their antioxidant activity was enhanced. Novelty and scientific contribution. Tomato fruits have been the subject of numerous studies; however, functional compound extraction through environmentally friendly methods remains an attractive use of native tomato fruit, enhancing its limited production and harnessing a large amount of tomato product industry. There are few reports where environmentally friendly extraction methods are combined; even rarer are those where green solvents are also used. In this work, the combination of different environmentally friendly extraction methods improved the extraction of carotenoids and tocopherols and allowed to establish a more efficient process. These results could stimulate the use of clean technologies and make the native tomato more attractive for industrial or compound extraction processes.https://hrcak.srce.hr/file/398542lipophilic antioxidantsnative tomato genotypeenzyme-assisted extractionsonicationgreen solvents |
| spellingShingle | Darío R. Gómez-Linton Arturo Navarro-Ocaña Silvestre Alavez Ricardo Lobato-Ortiz Angélica Román-Guerrero José Alberto Mendoza-Espinoza Juan Manuel Villa-Hernández Laura J. Pérez-Flores Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies Food Technology and Biotechnology lipophilic antioxidants native tomato genotype enzyme-assisted extraction sonication green solvents |
| title | Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies |
| title_full | Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies |
| title_fullStr | Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies |
| title_full_unstemmed | Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies |
| title_short | Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies |
| title_sort | extraction of lipophilic antioxidants from native tomato using green technologies |
| topic | lipophilic antioxidants native tomato genotype enzyme-assisted extraction sonication green solvents |
| url | https://hrcak.srce.hr/file/398542 |
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