The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian Amazon
This study tested the hypothesis that cocoa monoculture (MS) and cocoa-açai agroforestry systems (AFS) may influence the microbial community structure and populations of plant growth-promoting bacteria (PGPR). Accordingly, the aim was to analyze the microbial community structure and PGPR populations...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/2/398 |
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| author | Rosiane do Socorro dos Reis de Sousa Giulia Victória Silva Lima Josinete Torres Garcias Graziane de Oliveira Gomes Jackeline Rossetti Mateus Lucimar Di Paula dos Santos Madeira Lucy Seldin Hervé Louis Ghislain Rogez Joana Montezano Marques |
| author_facet | Rosiane do Socorro dos Reis de Sousa Giulia Victória Silva Lima Josinete Torres Garcias Graziane de Oliveira Gomes Jackeline Rossetti Mateus Lucimar Di Paula dos Santos Madeira Lucy Seldin Hervé Louis Ghislain Rogez Joana Montezano Marques |
| author_sort | Rosiane do Socorro dos Reis de Sousa |
| collection | DOAJ |
| description | This study tested the hypothesis that cocoa monoculture (MS) and cocoa-açai agroforestry systems (AFS) may influence the microbial community structure and populations of plant growth-promoting bacteria (PGPR). Accordingly, the aim was to analyze the microbial community structure and PGPR populations in different agroecosystems in the Brazilian Amazon. To achieve this, the rhizosphere microbial community of cocoa and açai plants in both Amazonian seasons (dry and rainy) was analyzed using culture-dependent (PGPR screening) and -independent methods [PCR-DGGE based on <i>rrs</i>, <i>alp</i>, <i>nif</i>H gene, and intergenic region (ITS) of fungi]. Concerning PGPR screening, out of 48 isolated bacterial strains, 25% were capable of siderophore production, 29% of mineralized organic phosphate, 8% of inorganic phosphate solubilization, and 4% of indole acetic acid production. Moreover, 17% of isolates could inhibit the growth of various phytopathogenic fungi. Statistical analyses of DGGE fingerprints (<i>p</i> < 0.05) showed that bacterial and fungal community structures in the rhizosphere were influenced by the seasons, supporting the results of the physicochemical analysis of the environment. Furthermore, as hypothesized, microbial communities differed statistically when comparing the MS and AFS. These findings provide important insights into the influence of climate and cultivation systems on soil microbial communities to guide the development of sustainable agricultural practices. |
| first_indexed | 2024-03-07T22:20:56Z |
| format | Article |
| id | doaj.art-789346946fa24668ace5df27720b966b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-07T22:20:56Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-789346946fa24668ace5df27720b966b2024-02-23T15:28:25ZengMDPI AGMicroorganisms2076-26072024-02-0112239810.3390/microorganisms12020398The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian AmazonRosiane do Socorro dos Reis de Sousa0Giulia Victória Silva Lima1Josinete Torres Garcias2Graziane de Oliveira Gomes3Jackeline Rossetti Mateus4Lucimar Di Paula dos Santos Madeira5Lucy Seldin6Hervé Louis Ghislain Rogez7Joana Montezano Marques8Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, BrazilInstituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, BrazilInstituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, BrazilInstituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, BrazilInstituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Rio de Janeiro, BrazilInstituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, BrazilInstituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Rio de Janeiro, BrazilInstituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, BrazilInstituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, BrazilThis study tested the hypothesis that cocoa monoculture (MS) and cocoa-açai agroforestry systems (AFS) may influence the microbial community structure and populations of plant growth-promoting bacteria (PGPR). Accordingly, the aim was to analyze the microbial community structure and PGPR populations in different agroecosystems in the Brazilian Amazon. To achieve this, the rhizosphere microbial community of cocoa and açai plants in both Amazonian seasons (dry and rainy) was analyzed using culture-dependent (PGPR screening) and -independent methods [PCR-DGGE based on <i>rrs</i>, <i>alp</i>, <i>nif</i>H gene, and intergenic region (ITS) of fungi]. Concerning PGPR screening, out of 48 isolated bacterial strains, 25% were capable of siderophore production, 29% of mineralized organic phosphate, 8% of inorganic phosphate solubilization, and 4% of indole acetic acid production. Moreover, 17% of isolates could inhibit the growth of various phytopathogenic fungi. Statistical analyses of DGGE fingerprints (<i>p</i> < 0.05) showed that bacterial and fungal community structures in the rhizosphere were influenced by the seasons, supporting the results of the physicochemical analysis of the environment. Furthermore, as hypothesized, microbial communities differed statistically when comparing the MS and AFS. These findings provide important insights into the influence of climate and cultivation systems on soil microbial communities to guide the development of sustainable agricultural practices.https://www.mdpi.com/2076-2607/12/2/398agroforestry systembacterial and fungal communitiesPGPR |
| spellingShingle | Rosiane do Socorro dos Reis de Sousa Giulia Victória Silva Lima Josinete Torres Garcias Graziane de Oliveira Gomes Jackeline Rossetti Mateus Lucimar Di Paula dos Santos Madeira Lucy Seldin Hervé Louis Ghislain Rogez Joana Montezano Marques The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian Amazon Microorganisms agroforestry system bacterial and fungal communities PGPR |
| title | The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian Amazon |
| title_full | The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian Amazon |
| title_fullStr | The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian Amazon |
| title_full_unstemmed | The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian Amazon |
| title_short | The Microbial Community Structure in the Rhizosphere of <i>Theobroma cacao</i> L. and <i>Euterpe oleracea</i> Mart. Is Influenced by Agriculture System in the Brazilian Amazon |
| title_sort | microbial community structure in the rhizosphere of i theobroma cacao i l and i euterpe oleracea i mart is influenced by agriculture system in the brazilian amazon |
| topic | agroforestry system bacterial and fungal communities PGPR |
| url | https://www.mdpi.com/2076-2607/12/2/398 |
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