A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos

A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos

Abstract A wide-field microscope with epi-fluorescence and selective plane illumination was combined with a single-photon avalanche diode (SPAD) array camera to enable live-cell fluorescence lifetime imaging (FLIM) using time-correlated single-photon counting (TCSPC). The camera sensor comprised of...

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Bibliographic Details
Main Authors: Jakub Nedbal, Francesco Mattioli Della Rocca, Iveta T. Ivanova, Andrew Allan, Jeremy Graham, Richard Walker, Robert K. Henderson, Klaus Suhling
Format: Article
Language:English
Published: Nature Portfolio 2024-03-01
Series:Scientific Reports
Subjects:
Single photon avalanche diode (SPAD)
Nonlinearity correction
Fluorescence lifetime imaging (FLIM)
Selective-plane illumination microscopy
Algae
Giant unilamellar vesicle (GUV)
Online Access:https://doi.org/10.1038/s41598-024-56122-1

Internet

https://doi.org/10.1038/s41598-024-56122-1

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