Identification of critical chemical modifications by size exclusion chromatography of stressed antibody-target complexes with competitive binding

Identification of critical chemical modifications by size exclusion chromatography of stressed antibody-target complexes with competitive binding

Chemical modifications (attributes) in the binding regions of stressed therapeutic proteins may affect binding to target and efficacy of therapeutic proteins. The method presented here describes the criticality assessment of therapeutic antibody modifications by size-exclusion chromatography (SEC) o...

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Bibliographic Details
Main Authors: Rachel Liuqing Shi, Gang Xiao, Thomas M. Dillon, Arnold McAuley, Margaret S. Ricci, Pavel V. Bondarenko
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:mAbs
Subjects:
Monoclonal antibody
size exclusion chromatography
mass spectrometry
critical attribute assessment
competitive binding
antibody-antigen binding
Online Access:https://www.tandfonline.com/doi/10.1080/19420862.2021.1887612

Internet

https://www.tandfonline.com/doi/10.1080/19420862.2021.1887612

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