The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i>
Infecting bacteriophage T4 uses a contractile tail structure to breach the envelope of the <i>Escherichia coli</i> host cell. During contraction, the tail tube headed with the “central spike complex” is thought to mechanically puncture the outer membrane. We show here that a purified tip...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/10/1135 |
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| author | Sabrina Wenzel Mikhail M. Shneider Petr G. Leiman Andreas Kuhn Dorothee Kiefer |
| author_facet | Sabrina Wenzel Mikhail M. Shneider Petr G. Leiman Andreas Kuhn Dorothee Kiefer |
| author_sort | Sabrina Wenzel |
| collection | DOAJ |
| description | Infecting bacteriophage T4 uses a contractile tail structure to breach the envelope of the <i>Escherichia coli</i> host cell. During contraction, the tail tube headed with the “central spike complex” is thought to mechanically puncture the outer membrane. We show here that a purified tip fragment of the central spike complex interacts with periplasmic chaperone PpiD, which is anchored to the cytoplasmic membrane. PpiD may be involved in the penetration of the inner membrane by the T4 injection machinery, resulting in a DNA-conducting channel to translocate the phage DNA into the interior of the cell. Host cells with the <i>ppiD</i> gene deleted showed partial reduction in the plating efficiency of T4, suggesting a supporting role of PpiD to improve the efficiency of the infection process. |
| first_indexed | 2024-03-10T15:48:52Z |
| format | Article |
| id | doaj.art-78bb2135848b474481e6eafbe0077fd1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T15:48:52Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-78bb2135848b474481e6eafbe0077fd12023-11-20T16:14:27ZengMDPI AGViruses1999-49152020-10-011210113510.3390/v12101135The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i>Sabrina Wenzel0Mikhail M. Shneider1Petr G. Leiman2Andreas Kuhn3Dorothee Kiefer4Institute of Biology, University of Hohenheim, 190h, Garbenstr. 30, 70599 Stuttgart, GermanyShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Laboratory of Molecular Bioengineering, 16/10 Miklukho-Maklaya St., 117997 Moscow, RussiaDepartment of BMB, Basic Sciences Building 6.600D, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0647, USAInstitute of Biology, University of Hohenheim, 190h, Garbenstr. 30, 70599 Stuttgart, GermanyInstitute of Biology, University of Hohenheim, 190h, Garbenstr. 30, 70599 Stuttgart, GermanyInfecting bacteriophage T4 uses a contractile tail structure to breach the envelope of the <i>Escherichia coli</i> host cell. During contraction, the tail tube headed with the “central spike complex” is thought to mechanically puncture the outer membrane. We show here that a purified tip fragment of the central spike complex interacts with periplasmic chaperone PpiD, which is anchored to the cytoplasmic membrane. PpiD may be involved in the penetration of the inner membrane by the T4 injection machinery, resulting in a DNA-conducting channel to translocate the phage DNA into the interior of the cell. Host cells with the <i>ppiD</i> gene deleted showed partial reduction in the plating efficiency of T4, suggesting a supporting role of PpiD to improve the efficiency of the infection process.https://www.mdpi.com/1999-4915/12/10/1135bacteriophage T4tail structureperiplasmic chaperoneDNA translocation |
| spellingShingle | Sabrina Wenzel Mikhail M. Shneider Petr G. Leiman Andreas Kuhn Dorothee Kiefer The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i> Viruses bacteriophage T4 tail structure periplasmic chaperone DNA translocation |
| title | The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i> |
| title_full | The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i> |
| title_fullStr | The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i> |
| title_full_unstemmed | The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i> |
| title_short | The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of <i>Escherichia coli</i> |
| title_sort | central spike complex of bacteriophage t4 contacts ppid in the periplasm of i escherichia coli i |
| topic | bacteriophage T4 tail structure periplasmic chaperone DNA translocation |
| url | https://www.mdpi.com/1999-4915/12/10/1135 |
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