Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology

Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology

Primary hepatocytes are a major tool in biomedical research. However, obtaining high yields of variable hepatocytes is technically challenging. Most protocols rely on the two-step collagenase perfusion protocol introduced by Per Ottar Seglen in 1976. In this procedure, the liver is perfused in situ...

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Bibliographic Details
Main Authors: Carsten Poggel, Timo Adams, Ronald Janzen, Alexander Hofmann, Olaf Hardt, Elke Roeb, Sarah K. Schröder, Carmen G. Tag, Martin Roderfeld, Ralf Weiskirchen
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Biomedicines
Subjects:
primary liver cells
hepatocyte
automated isolation
cell separation
cell culture
perfusion
Online Access:https://www.mdpi.com/2227-9059/10/9/2198

Internet

https://www.mdpi.com/2227-9059/10/9/2198

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