A Model System for Sensitive Detection of Viable <i>E. coli</i> Bacteria Combining Direct Viability PCR and a Novel Microarray-Based Detection Approach

A Model System for Sensitive Detection of Viable <i>E. coli</i> Bacteria Combining Direct Viability PCR and a Novel Microarray-Based Detection Approach

We established an innovative approach that included direct, viability, and nested PCR for rapid and reliable identification of the fecal indicator organism <i>Escherichia coli</i> (<i>E. coli</i>). Direct PCR enabled successful amplification of the target <i>uidA</i&...

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Bibliographic Details
Main Authors: Lydia Lehniger, Anne Rudloff, Sibyll Pollok, Norman Große, Kristin Wessel, Monique Brendel, Jürgen Popp, Karina Weber
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Chemosensors
Subjects:
<i>E. coli</i>
water monitoring
direct viability nested PCR
microarray-based DNA hybridization
Online Access:https://www.mdpi.com/2227-9040/9/12/357

Internet

https://www.mdpi.com/2227-9040/9/12/357

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