One-Enzyme RTX-PCR for the Detection of RNA Viruses from Multiple Virus Genera and Crop Plants

One-Enzyme RTX-PCR for the Detection of RNA Viruses from Multiple Virus Genera and Crop Plants

Reverse transcription PCR (RT-PCR) is a popular method for detecting RNA viruses in plants. RT-PCR is usually performed in a classical two-step procedure: in the first step, cDNA is synthesized by reverse transcriptase (RT), followed by PCR amplification by a thermostable polymerase in a separate tu...

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Bibliographic Details
Main Authors: Hana Hoffmeisterová, Kateřina Kratochvílová, Noemi Čeřovská, Lucie Slavíková, Jakub Dušek, Karel Muller, Jan Fousek, Helena Plchová, Oldřich Navrátil, Jiban Kumar Kundu, Tomáš Moravec
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Viruses
Subjects:
virus detection
Tobamovirus
Potyvirus
Polerovirus
Luteovirus
Potexvirus
Online Access:https://www.mdpi.com/1999-4915/14/2/298

Internet

https://www.mdpi.com/1999-4915/14/2/298

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