Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes.
In order for eukaryotic cells to function properly, they must establish polarity. The Drosophila oocyte uses mRNA localization to establish polarity and hence provides a genetically tractable model in which to study this process. The spatial restriction of oskar mRNA and its subsequent protein produ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3823658?pdf=render |
| _version_ | 1831750098291261440 |
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| author | Paulomi Sanghavi Shobha Laxani Xuan Li Simon L Bullock Graydon B Gonsalvez |
| author_facet | Paulomi Sanghavi Shobha Laxani Xuan Li Simon L Bullock Graydon B Gonsalvez |
| author_sort | Paulomi Sanghavi |
| collection | DOAJ |
| description | In order for eukaryotic cells to function properly, they must establish polarity. The Drosophila oocyte uses mRNA localization to establish polarity and hence provides a genetically tractable model in which to study this process. The spatial restriction of oskar mRNA and its subsequent protein product is necessary for embryonic patterning. The localization of oskar mRNA requires microtubules and microtubule-based motor proteins. Null mutants in Kinesin heavy chain (Khc), the motor subunit of the plus end-directed Kinesin-1, result in oskar mRNA delocalization. Although the majority of oskar particles are non-motile in khc nulls, a small fraction of particles display active motility. Thus, a motor other than Kinesin-1 could conceivably also participate in oskar mRNA localization. Here we show that Dynein heavy chain (Dhc), the motor subunit of the minus end-directed Dynein complex, extensively co-localizes with Khc and oskar mRNA. In addition, immunoprecipitation of the Dynein complex specifically co-precipitated oskar mRNA and Khc. Lastly, germline-specific depletion of Dhc resulted in oskar mRNA and Khc delocalization. Our results therefore suggest that efficient posterior localization of oskar mRNA requires the concerted activities of both Dynein and Kinesin-1. |
| first_indexed | 2024-12-21T22:21:42Z |
| format | Article |
| id | doaj.art-7942cd1788d6450da9f0d84ace1449d2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:21:42Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-7942cd1788d6450da9f0d84ace1449d22022-12-21T18:48:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e8060510.1371/journal.pone.0080605Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes.Paulomi SanghaviShobha LaxaniXuan LiSimon L BullockGraydon B GonsalvezIn order for eukaryotic cells to function properly, they must establish polarity. The Drosophila oocyte uses mRNA localization to establish polarity and hence provides a genetically tractable model in which to study this process. The spatial restriction of oskar mRNA and its subsequent protein product is necessary for embryonic patterning. The localization of oskar mRNA requires microtubules and microtubule-based motor proteins. Null mutants in Kinesin heavy chain (Khc), the motor subunit of the plus end-directed Kinesin-1, result in oskar mRNA delocalization. Although the majority of oskar particles are non-motile in khc nulls, a small fraction of particles display active motility. Thus, a motor other than Kinesin-1 could conceivably also participate in oskar mRNA localization. Here we show that Dynein heavy chain (Dhc), the motor subunit of the minus end-directed Dynein complex, extensively co-localizes with Khc and oskar mRNA. In addition, immunoprecipitation of the Dynein complex specifically co-precipitated oskar mRNA and Khc. Lastly, germline-specific depletion of Dhc resulted in oskar mRNA and Khc delocalization. Our results therefore suggest that efficient posterior localization of oskar mRNA requires the concerted activities of both Dynein and Kinesin-1.http://europepmc.org/articles/PMC3823658?pdf=render |
| spellingShingle | Paulomi Sanghavi Shobha Laxani Xuan Li Simon L Bullock Graydon B Gonsalvez Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. PLoS ONE |
| title | Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. |
| title_full | Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. |
| title_fullStr | Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. |
| title_full_unstemmed | Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. |
| title_short | Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. |
| title_sort | dynein associates with oskar mrnps and is required for their efficient net plus end localization in drosophila oocytes |
| url | http://europepmc.org/articles/PMC3823658?pdf=render |
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