Immune response and antioxidant status of broilers as influenced by oxidized vegetable oil and pomegranate peel
The experiment was designed as a 3 × 3 × 2 factorial arrangement of treatments, including (i) pomegranate peel (zero, 4%, and 8 percent), (ii) oxidized soybean oil (zero, 2%, and 4 percent), and (iii) alpha-tocopherol (zero and 200 mg/kg)....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Animal Sciences and Technology
2021-09-01
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| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?pid=jast-63-5-1034 |
| Summary: | The experiment was designed as a 3 × 3 × 2 factorial arrangement of
treatments, including (i) pomegranate peel (zero, 4%, and 8 percent), (ii)
oxidized soybean oil (zero, 2%, and 4 percent), and (iii) alpha-tocopherol (zero
and 200 mg/kg). Supplementation of 8% pomegranate peel in diets significantly
decreased the growth performance of broiler chickens. The supplementation of 4%
oxidized oil in diets significantly reduced body weight gain and Feed intake
whole experimental period (p < 0.05). The results showed
that supplementation of 4% pomegranate peel in the diet was associated with low
aspartate transaminase (AST), alanine transaminase, and malondialdehyde (MDA).
However, 4% pomegranate peel increased the total antioxidant capacity (TAC) and
superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. The
supplemental 4% oxidized oil increased the serum AST, alanine aminotransferase
(ALT), and MDA concentrations. TAC, SOD, and Catalase (CAT) activities were
affected by 4% oxidized oil and alpha-tocopherol. The use of oxidized oil and
vitamin E decreased MDA concentration. The serum glucose and globulin
concentrations were significantly lower in the 8% pomegranate peel. The results
showed that supplementation with 4% pomegranate peel in diets reduced serum
low-density lipoprotein (LDL). The inclusion of 4% oxidized oil in diets reduced
serum glucose and increased the blood lipid concentration such as triglyceride,
cholesterol and LDL. Vitamin E supplementation reduced the serum cholesterol and
LDL concentrations. The use of 8% pomegranate peel reduced red blood cell (RBC),
hemoglobin, and packed cell value (PCV). The results indicated that
supplementation with 8% pomegranate peel and 4% oxidized oil in diets decreased
the immunoglobulin concentration in broilers. In addition, it was found that the
inclusion of 4% pomegranate peel in diets resulted in higher IgG, IgM and total
immunoglobulin. Pomegranate peel supplementation significantly decreased meat
MDA concentration. Supplementation of 4% oxidized oil increased MDA of meat
(p < 0.05). Vitamin E supplementation (200 mg/kg)
significantly decreased MDA of meat (p < 0.05).
Consequently, the results of this experiment showed that supplementation with 4%
pomegranate peel had beneficial effects on broiler chickens. It was also found
that feeding 2% oxidized oil in diets had no adverse effect on broilers. |
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| ISSN: | 2672-0191 2055-0391 |