Enhancement of the Thermostability of <i>Microbacterium</i> Esterase by Combinatorial Rational Design
The esterase EstSIT01 from <i>Microbacterium</i> can catalyze the asymmetric hydrolysis of <i>meso</i>-dimethyl ester to produce the crucial chiral intermediate (4<i>S</i>, 5<i>R</i>)-hemimethyl ester for <i>d</i>-biotin synthesis. Despite...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2024-12-01
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Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/29/24/5839 |