Enhancement of the Thermostability of <i>Microbacterium</i> Esterase by Combinatorial Rational Design

The esterase EstSIT01 from <i>Microbacterium</i> can catalyze the asymmetric hydrolysis of <i>meso</i>-dimethyl ester to produce the crucial chiral intermediate (4<i>S</i>, 5<i>R</i>)-hemimethyl ester for <i>d</i>-biotin synthesis. Despite...

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Bibliographic Details
Main Authors: Wenyu Peng, Xiaomei Wu, Baodi Ma, Yi Xu
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/29/24/5839