ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL
Histological and microbiological comparative analysis of bovine pericardium preserved for 30 and 60 days in glycerin (98%), supersaturated sugar solution (300%), supersaturated solution of salt (150%) and unprocessed honey were performed aiming to establish alternative ways of preservation of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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EDUFU
2014-07-01
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| Series: | Veterinary News |
| Subjects: | |
| Online Access: | http://www.seer.ufu.br/index.php/vetnot/article/view/27433/16100 |
| _version_ | 1811322353582342144 |
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| author | Antônio Fernando Bariani Júnior Gabriela Castro Lopes Ana Rosa Crisci Rubens Ferracini Júnior Camila Souza de Oliveira Guimarães Gregório Corrêa Guimarães |
| author_facet | Antônio Fernando Bariani Júnior Gabriela Castro Lopes Ana Rosa Crisci Rubens Ferracini Júnior Camila Souza de Oliveira Guimarães Gregório Corrêa Guimarães |
| author_sort | Antônio Fernando Bariani Júnior |
| collection | DOAJ |
| description | Histological and microbiological
comparative analysis of bovine
pericardium preserved for 30 and 60 days
in glycerin (98%), supersaturated sugar
solution (300%), supersaturated solution
of salt (150%) and unprocessed honey
were performed aiming to establish
alternative ways of preservation of
biological membranes. Samples of
pericardium from five cattle were collected
in fridge and maintained for up to 60 days
in containers with the above mentioned
solutions of conservation. For
morphological analysis of the constituents
of bovine pericardium were performed
routine histological techniques associated
to two microbiological culture techniques:
streaking on the surface of the plate and
pour-plate. The supersaturated solution of
sugar (300%) was the medium of
preservation with results closer to the
obtained with glycerin (98%), but was
unable to prevent microorganisms growth.
The unprocessed honey and
supersaturated solution of salt (150%) had
suitable antiseptic characteristics, but
failed to effectively conserve the
pericardium, with a greater structural
disorganization of the connective tissue,
indicating advanced process of autolysis
after 60 days of storage. It was concluded
that glycerin (98%) appeared as the most
efficient solution when compared to the
others evaluated in this study. |
| first_indexed | 2024-04-13T13:33:49Z |
| format | Article |
| id | doaj.art-797c9f905648474887a5525401194032 |
| institution | Directory Open Access Journal |
| issn | 1983-0777 1983-0777 |
| language | English |
| last_indexed | 2024-04-13T13:33:49Z |
| publishDate | 2014-07-01 |
| publisher | EDUFU |
| record_format | Article |
| series | Veterinary News |
| spelling | doaj.art-797c9f905648474887a55254011940322022-12-22T02:44:50ZengEDUFUVeterinary News1983-07771983-07772014-07-0120212410.14393/VTv20n2a2014.27433 ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SALAntônio Fernando Bariani Júnior 0Gabriela Castro Lopes1Ana Rosa Crisci2Rubens Ferracini Júnior 3Camila Souza de Oliveira Guimarães4Gregório Corrêa Guimarães5Centro Universitário Barão de MauáUniversidade Federal de LavrasCentro Universitário Barão de MauáCentro Universitário Barão de MauáUniversidade Federal do Triângulo Mineiro Universidade Federal de LavrasHistological and microbiological comparative analysis of bovine pericardium preserved for 30 and 60 days in glycerin (98%), supersaturated sugar solution (300%), supersaturated solution of salt (150%) and unprocessed honey were performed aiming to establish alternative ways of preservation of biological membranes. Samples of pericardium from five cattle were collected in fridge and maintained for up to 60 days in containers with the above mentioned solutions of conservation. For morphological analysis of the constituents of bovine pericardium were performed routine histological techniques associated to two microbiological culture techniques: streaking on the surface of the plate and pour-plate. The supersaturated solution of sugar (300%) was the medium of preservation with results closer to the obtained with glycerin (98%), but was unable to prevent microorganisms growth. The unprocessed honey and supersaturated solution of salt (150%) had suitable antiseptic characteristics, but failed to effectively conserve the pericardium, with a greater structural disorganization of the connective tissue, indicating advanced process of autolysis after 60 days of storage. It was concluded that glycerin (98%) appeared as the most efficient solution when compared to the others evaluated in this study.http://www.seer.ufu.br/index.php/vetnot/article/view/27433/16100Preservation methodsPericardiumBiological membrane |
| spellingShingle | Antônio Fernando Bariani Júnior Gabriela Castro Lopes Ana Rosa Crisci Rubens Ferracini Júnior Camila Souza de Oliveira Guimarães Gregório Corrêa Guimarães ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL Veterinary News Preservation methods Pericardium Biological membrane |
| title | ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL |
| title_full | ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL |
| title_fullStr | ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL |
| title_full_unstemmed | ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL |
| title_short | ANÁLISE MORFOLÓGICA E MICROBIOLÓGICA DO PERICÁRDIO BOVINO CONSERVADO EM AÇÚCAR, GLICERINA, MEL E SAL |
| title_sort | analise morfologica e microbiologica do pericardio bovino conservado em acucar glicerina mel e sal |
| topic | Preservation methods Pericardium Biological membrane |
| url | http://www.seer.ufu.br/index.php/vetnot/article/view/27433/16100 |
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