Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases

Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and off-target effects. CRISPR delivered as ribonuc...

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Bibliographic Details
Main Authors: Yingxiao Zhang, Yanhao Cheng, Hong Fang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, Randall P. Niedz, James N. Culver, Yiping Qi
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Genome Editing
Subjects:
CRISPR-Cas12a
ribonucleoprotein
genome editing
rice
citrus
protoplast
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2022.780238/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2022.780238/full

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