Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9

Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9

ABSTRACT CRISPR/Cas9 genome editing technology has been implemented in almost all living organisms. Its editing precision appears to be very high and therefore could represent a big change from conventional genetic engineering approaches. However, guide RNA binding to nucleotides similar to the targ...

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Bibliographic Details
Main Authors: Sandra Garrigues, Mao Peng, Roland S. Kun, Ronald P. de Vries
Format: Article
Language:English
Published: American Society for Microbiology 2023-08-01
Series:mBio
Subjects:
Aspergillus niger
biosafety
CRISPR-Cas9
risk assessment
ΔkusA
Online Access:https://journals.asm.org/doi/10.1128/mbio.00668-23

Internet

https://journals.asm.org/doi/10.1128/mbio.00668-23

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