Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates
In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-10-01
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| Series: | Biosensors |
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| Online Access: | http://www.mdpi.com/2079-6374/5/4/664 |
| _version_ | 1818065135596470272 |
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| author | Peter Owens Nigel Phillipson Jayakumar Perumal Gerard M. O’Connor Malini Olivo |
| author_facet | Peter Owens Nigel Phillipson Jayakumar Perumal Gerard M. O’Connor Malini Olivo |
| author_sort | Peter Owens |
| collection | DOAJ |
| description | In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein–antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration. |
| first_indexed | 2024-12-10T14:47:05Z |
| format | Article |
| id | doaj.art-798fd5748b6d4dabbe2df0c9154ce727 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-10T14:47:05Z |
| publishDate | 2015-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-798fd5748b6d4dabbe2df0c9154ce7272022-12-22T01:44:33ZengMDPI AGBiosensors2079-63742015-10-015466467710.3390/bios5040664bios5040664Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS SubstratesPeter Owens0Nigel Phillipson1Jayakumar Perumal2Gerard M. O’Connor3Malini Olivo4Centre for Microscopy and Imaging, National University Ireland, University Road, Galway, IrelandSchool of Physics, National University Ireland, University Road, Galway, IrelandBio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios 138667, SingaporeSchool of Physics, National University Ireland, University Road, Galway, IrelandSchool of Physics, National University Ireland, University Road, Galway, IrelandIn this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein–antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.http://www.mdpi.com/2079-6374/5/4/664Raman spectroscopyprotein sensingsurface-enhanced Raman scatteringbiomarker |
| spellingShingle | Peter Owens Nigel Phillipson Jayakumar Perumal Gerard M. O’Connor Malini Olivo Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates Biosensors Raman spectroscopy protein sensing surface-enhanced Raman scattering biomarker |
| title | Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates |
| title_full | Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates |
| title_fullStr | Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates |
| title_full_unstemmed | Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates |
| title_short | Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates |
| title_sort | sensing of p53 and egfr biomarkers using high efficiency sers substrates |
| topic | Raman spectroscopy protein sensing surface-enhanced Raman scattering biomarker |
| url | http://www.mdpi.com/2079-6374/5/4/664 |
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