Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep
An isotope dilution method of [U-13C]glucose and [l-13C]leucine was simultaneously conducted to determine the effect of grinding process of steam-rolled barley grain on rates of plasma glucose and leucine turnover and whole-body protein synthesis (WBPS) in sheep. Six adult sheep were assigned to two...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2017.1383259 |
| _version_ | 1818032089201639424 |
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| author | Hiroaki Sano Yuko Saito Mohammad Al-Mamun |
| author_facet | Hiroaki Sano Yuko Saito Mohammad Al-Mamun |
| author_sort | Hiroaki Sano |
| collection | DOAJ |
| description | An isotope dilution method of [U-13C]glucose and [l-13C]leucine was simultaneously conducted to determine the effect of grinding process of steam-rolled barley grain on rates of plasma glucose and leucine turnover and whole-body protein synthesis (WBPS) in sheep. Six adult sheep were assigned to two dietary treatments and the experiment utilized a crossover design for two 21-day periods. The experimental diet was consisted of mixed hay and steam-rolled barley grain (1:1 ratio). Barley grain was treated either with or without grinding (GR diet and SR diet, respectively). Sheep were fed isoenergetically and isonitrogenously in both the treatments. The primed-continuous infusion method of [U-13C]glucose and [1-13C]leucine was simultaneously performed on day 21. The pH and ammonia concentrations in rumen fluid decreased (P < 0.05) and concentrations of total VFA, acetate, propionate and n-butyrate increased (P < 0.05) after feeding. Rates of plasma glucose and leucine turnover and WBPS did not differ between the dietary treatments. It is likely that grinding process of steam-rolled barley grain did not influence plasma glucose and leucine kinetics and WBPS in sheep fed the diets of mixed hay and barley grain at 1:1 ratio. |
| first_indexed | 2024-12-10T06:01:49Z |
| format | Article |
| id | doaj.art-79b21eb8b75b45f892039860d2679e51 |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-12-10T06:01:49Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-79b21eb8b75b45f892039860d2679e512022-12-22T01:59:47ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442018-01-0146169670110.1080/09712119.2017.13832591383259Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheepHiroaki Sano0Yuko Saito1Mohammad Al-Mamun2Iwate UniversityIwate UniversityIwate UniversityAn isotope dilution method of [U-13C]glucose and [l-13C]leucine was simultaneously conducted to determine the effect of grinding process of steam-rolled barley grain on rates of plasma glucose and leucine turnover and whole-body protein synthesis (WBPS) in sheep. Six adult sheep were assigned to two dietary treatments and the experiment utilized a crossover design for two 21-day periods. The experimental diet was consisted of mixed hay and steam-rolled barley grain (1:1 ratio). Barley grain was treated either with or without grinding (GR diet and SR diet, respectively). Sheep were fed isoenergetically and isonitrogenously in both the treatments. The primed-continuous infusion method of [U-13C]glucose and [1-13C]leucine was simultaneously performed on day 21. The pH and ammonia concentrations in rumen fluid decreased (P < 0.05) and concentrations of total VFA, acetate, propionate and n-butyrate increased (P < 0.05) after feeding. Rates of plasma glucose and leucine turnover and WBPS did not differ between the dietary treatments. It is likely that grinding process of steam-rolled barley grain did not influence plasma glucose and leucine kinetics and WBPS in sheep fed the diets of mixed hay and barley grain at 1:1 ratio.http://dx.doi.org/10.1080/09712119.2017.1383259Amino acid metabolismbarley grainglucose metabolismprocessingsheep |
| spellingShingle | Hiroaki Sano Yuko Saito Mohammad Al-Mamun Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep Journal of Applied Animal Research Amino acid metabolism barley grain glucose metabolism processing sheep |
| title | Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep |
| title_full | Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep |
| title_fullStr | Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep |
| title_full_unstemmed | Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep |
| title_short | Effect of grinding process of steam-rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep |
| title_sort | effect of grinding process of steam rolled barley grain on plasma glucose and leucine kinetics and protein synthesis in sheep |
| topic | Amino acid metabolism barley grain glucose metabolism processing sheep |
| url | http://dx.doi.org/10.1080/09712119.2017.1383259 |
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