Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice
Vaccines are one of the most effective means of preventing influenza A, typically containing the hemagglutinin (HA) of the influenza A virus. However, antigenic drift and shift of the influenza A virus can lead to instability in vaccine efficacy. Compared to HA, the antigenic variation rate of neura...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/12/3/300 |
| _version_ | 1797239102794366976 |
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| author | Mingyang Li Mengyuan Liu Shaohui Song Ruirui Zhao Yun Xie Jing Liu Lilan Xu Xuefeng Ma Mingyu Song Jian Zhou Guoyang Liao |
| author_facet | Mingyang Li Mengyuan Liu Shaohui Song Ruirui Zhao Yun Xie Jing Liu Lilan Xu Xuefeng Ma Mingyu Song Jian Zhou Guoyang Liao |
| author_sort | Mingyang Li |
| collection | DOAJ |
| description | Vaccines are one of the most effective means of preventing influenza A, typically containing the hemagglutinin (HA) of the influenza A virus. However, antigenic drift and shift of the influenza A virus can lead to instability in vaccine efficacy. Compared to HA, the antigenic variation rate of neuraminidase (NA) is slower. In traditional inactivated influenza vaccines, although they contain a certain amount of NA, there are significant differences between different batches, which cannot consistently induce NA-based immune responses. Therefore, NA is often overlooked in vaccine development. In this study, we report an mRNA vaccine encoding the NA of two strains of influenza A virus. The experimental results demonstrated that when matched with the viral strain, this mRNA vaccine induced high levels of neutralizing antibodies, providing a protective effect to mice in viral challenge experiments, and this immune response was shown to be biased towards the Th1 type. In summary, this study demonstrates that NA is a promising potential antigen, providing new insights for the development of influenza A virus vaccines. |
| first_indexed | 2024-04-24T17:46:12Z |
| format | Article |
| id | doaj.art-79b9c747288147b8a08bf639289aac49 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-24T17:46:12Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-79b9c747288147b8a08bf639289aac492024-03-27T14:07:04ZengMDPI AGVaccines2076-393X2024-03-0112330010.3390/vaccines12030300Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in MiceMingyang Li0Mengyuan Liu1Shaohui Song2Ruirui Zhao3Yun Xie4Jing Liu5Lilan Xu6Xuefeng Ma7Mingyu Song8Jian Zhou9Guoyang Liao10Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, ChinaVaccines are one of the most effective means of preventing influenza A, typically containing the hemagglutinin (HA) of the influenza A virus. However, antigenic drift and shift of the influenza A virus can lead to instability in vaccine efficacy. Compared to HA, the antigenic variation rate of neuraminidase (NA) is slower. In traditional inactivated influenza vaccines, although they contain a certain amount of NA, there are significant differences between different batches, which cannot consistently induce NA-based immune responses. Therefore, NA is often overlooked in vaccine development. In this study, we report an mRNA vaccine encoding the NA of two strains of influenza A virus. The experimental results demonstrated that when matched with the viral strain, this mRNA vaccine induced high levels of neutralizing antibodies, providing a protective effect to mice in viral challenge experiments, and this immune response was shown to be biased towards the Th1 type. In summary, this study demonstrates that NA is a promising potential antigen, providing new insights for the development of influenza A virus vaccines.https://www.mdpi.com/2076-393X/12/3/300mRNA vaccineinfluenza AneuraminidaseTh1 |
| spellingShingle | Mingyang Li Mengyuan Liu Shaohui Song Ruirui Zhao Yun Xie Jing Liu Lilan Xu Xuefeng Ma Mingyu Song Jian Zhou Guoyang Liao Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice Vaccines mRNA vaccine influenza A neuraminidase Th1 |
| title | Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice |
| title_full | Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice |
| title_fullStr | Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice |
| title_full_unstemmed | Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice |
| title_short | Influenza a Neuraminidase-Based Bivalent mRNA Vaccine Induces Th1-Type Immune Response and Provides Protective Effects in Mice |
| title_sort | influenza a neuraminidase based bivalent mrna vaccine induces th1 type immune response and provides protective effects in mice |
| topic | mRNA vaccine influenza A neuraminidase Th1 |
| url | https://www.mdpi.com/2076-393X/12/3/300 |
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