CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast

CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast

Abstract Background The creation of arrays of yeast strains each encoding a different protein with constant tags is a powerful method for understanding how genes and their proteins control cell function. As genetic tools become more sophisticated there is a need to create custom libraries encoding p...

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Bibliographic Details
Main Authors: Lisa K. Berry, Grace Heredge Thomas, Peter H. Thorpe
Format: Article
Language:English
Published: BMC 2020-03-01
Series:BMC Genomics
Subjects:
CRISPR-Cas9
Yeast
Array
GFP collection
SPA
Tag switching
Online Access:http://link.springer.com/article/10.1186/s12864-020-6634-9

Internet

http://link.springer.com/article/10.1186/s12864-020-6634-9

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