A Simple Validated Method for the Estimation of Pepsin Activity in Microtiter Array for the INFOGEST Protocol

The INFOGEST protocol has been widely used as a static <i>in-vitro</i> simulation of gastrointestinal food digestion for bioaccessibility assessments on bioactive compounds. The standardization of the activity of several enzymes, such as pepsin, via UV-spectrophotometry of digested hemog...

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Bibliographic Details
Main Authors: Maximiliano Ramm, Bárbara Alarcón-Zapata, Juan Monsalves, Luis Bustamante
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/12/20/3851