Continuous Spectrophotometric Assay for β-Glucuronidase
A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-d-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01304rr02 |