Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant properties
This study investigated in vitro antidiabetic, anti-obesity, and antioxidant activities of chia seed protein hydrolysates (CSPHs) and flaxseed protein hydrolysates (FSPHs) generated using three food-grade proteases at the hydrolysis at intervals of 120 min for 6 h. The inhibitory potentials of CSPHs...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2023.1223884/full |
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| author | Priti Mudgil Feyisola Fisayo Ajayi Amani Alkaabi Maitha Alsubousi Brij Pal Singh Sajid Maqsood |
| author_facet | Priti Mudgil Feyisola Fisayo Ajayi Amani Alkaabi Maitha Alsubousi Brij Pal Singh Sajid Maqsood |
| author_sort | Priti Mudgil |
| collection | DOAJ |
| description | This study investigated in vitro antidiabetic, anti-obesity, and antioxidant activities of chia seed protein hydrolysates (CSPHs) and flaxseed protein hydrolysates (FSPHs) generated using three food-grade proteases at the hydrolysis at intervals of 120 min for 6 h. The inhibitory potentials of CSPHs and FSPHs on the enzymatic biomarkers related to diabetes (α-glucosidase and DPP-4) and obesity (pancreatic lipase and cholesteryl esterase) were determined. The antioxidant capacity of CSPHs and FSPHs was also assessed using ABTS, DPPH, and FRAP antioxidant assays. Increasing the proteolytic reaction time was shown to significantly increase the degree of hydrolysis values of CSPHs and FSPHs. Protein hydrolysates resulted in higher inhibitory potential against enzymatic biomarkers related to metabolic diseases (diabetes and obesity) as well as higher antioxidant activities compared with undigested proteins. Overall, the lowest IC50 inhibition values demonstrated in DPP-IV were observed by alcalase-catalyzed CSPH at 6 h (IC50 = 272.17 μg/mL) and bromelain-catalyzed FSPH at 6 h (IC50 = 338.70 μg/mL). The strongest inhibition of pancreatic lipase was observed in bromelain-catalyzed hydrolysates (IC50 = 292.43 μg/mL and IC50 = 307.62 μg/mL for CSPH and FSPH, respectively) after 6 h. These findings indicate that CSPHs and FSPHs exhibited enhanced antidiabetic, anti-obesity, and antioxidant properties. Therefore, these hydrolysates can be successfully used for their applications in the development of functional foods. |
| first_indexed | 2024-03-12T17:47:35Z |
| format | Article |
| id | doaj.art-79f631e5e35e410589a52b2e445d49a7 |
| institution | Directory Open Access Journal |
| issn | 2571-581X |
| language | English |
| last_indexed | 2024-03-12T17:47:35Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj.art-79f631e5e35e410589a52b2e445d49a72023-08-03T12:36:48ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2023-08-01710.3389/fsufs.2023.12238841223884Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant propertiesPriti MudgilFeyisola Fisayo AjayiAmani AlkaabiMaitha AlsubousiBrij Pal SinghSajid MaqsoodThis study investigated in vitro antidiabetic, anti-obesity, and antioxidant activities of chia seed protein hydrolysates (CSPHs) and flaxseed protein hydrolysates (FSPHs) generated using three food-grade proteases at the hydrolysis at intervals of 120 min for 6 h. The inhibitory potentials of CSPHs and FSPHs on the enzymatic biomarkers related to diabetes (α-glucosidase and DPP-4) and obesity (pancreatic lipase and cholesteryl esterase) were determined. The antioxidant capacity of CSPHs and FSPHs was also assessed using ABTS, DPPH, and FRAP antioxidant assays. Increasing the proteolytic reaction time was shown to significantly increase the degree of hydrolysis values of CSPHs and FSPHs. Protein hydrolysates resulted in higher inhibitory potential against enzymatic biomarkers related to metabolic diseases (diabetes and obesity) as well as higher antioxidant activities compared with undigested proteins. Overall, the lowest IC50 inhibition values demonstrated in DPP-IV were observed by alcalase-catalyzed CSPH at 6 h (IC50 = 272.17 μg/mL) and bromelain-catalyzed FSPH at 6 h (IC50 = 338.70 μg/mL). The strongest inhibition of pancreatic lipase was observed in bromelain-catalyzed hydrolysates (IC50 = 292.43 μg/mL and IC50 = 307.62 μg/mL for CSPH and FSPH, respectively) after 6 h. These findings indicate that CSPHs and FSPHs exhibited enhanced antidiabetic, anti-obesity, and antioxidant properties. Therefore, these hydrolysates can be successfully used for their applications in the development of functional foods.https://www.frontiersin.org/articles/10.3389/fsufs.2023.1223884/fullflaxseedchai seedprotein hydrolysatesantioxidantantidiabeticanti-obesity |
| spellingShingle | Priti Mudgil Feyisola Fisayo Ajayi Amani Alkaabi Maitha Alsubousi Brij Pal Singh Sajid Maqsood Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant properties Frontiers in Sustainable Food Systems flaxseed chai seed protein hydrolysates antioxidant antidiabetic anti-obesity |
| title | Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant properties |
| title_full | Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant properties |
| title_fullStr | Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant properties |
| title_full_unstemmed | Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant properties |
| title_short | Flaxseed- and chia seed-derived protein hydrolysates exhibiting enhanced in vitro antidiabetic, anti-obesity, and antioxidant properties |
| title_sort | flaxseed and chia seed derived protein hydrolysates exhibiting enhanced in vitro antidiabetic anti obesity and antioxidant properties |
| topic | flaxseed chai seed protein hydrolysates antioxidant antidiabetic anti-obesity |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2023.1223884/full |
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