Effects of Lower Temperature on Expression and Biochemical Characteristics of HCV NS3 Antigen Recombinant Protein

Effects of Lower Temperature on Expression and Biochemical Characteristics of HCV NS3 Antigen Recombinant Protein

The nonstructural antigen protein 3 of the hepatitis C virus (HCV NS3), commonly-used for HCV ELISA diagnosis, possesses protease and helicase activities. To prevent auto-degradation, a truncated NS3 protein was designed by removing the protease domain. Firstly, it was overexpressed in <i>E. c...

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Bibliographic Details
Main Authors: Chen-Ji Huang, Hwei-Ling Peng, Anil Kumar Patel, Reeta Rani Singhania, Cheng-Di Dong, Chih-Yu Cheng
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Catalysts
Subjects:
HCV
NS3
protein expression
diagnosis
helicase
protease
Online Access:https://www.mdpi.com/2073-4344/11/11/1297

Internet

https://www.mdpi.com/2073-4344/11/11/1297

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