Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein
Classical swine fever virus (CSFV) and porcine Circovirus type 2 (PCV2) are economically pivotal infectious disease viruses of swine. Alphaviral RNA replicon plasmids have been used as an important vector for constructing nucleic acid vaccines. Here, we aimed to construct a recombinant alphaviral pl...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1065532/full |
| _version_ | 1811185926793068544 |
|---|---|
| author | Fuyu Du Fuyu Du Zhi Cao Zixuan Ye Jun He Weijie Zhang Ke Zhang Pengbo Ning Pengbo Ning Pengbo Ning |
| author_facet | Fuyu Du Fuyu Du Zhi Cao Zixuan Ye Jun He Weijie Zhang Ke Zhang Pengbo Ning Pengbo Ning Pengbo Ning |
| author_sort | Fuyu Du |
| collection | DOAJ |
| description | Classical swine fever virus (CSFV) and porcine Circovirus type 2 (PCV2) are economically pivotal infectious disease viruses of swine. Alphaviral RNA replicon plasmids have been used as an important vector for constructing nucleic acid vaccines. Here, we aimed to construct a recombinant alphaviral plasmid vaccine pSCA1-E2-Erns-Cap-Rep for the prevention and control of CSFV and PCV2. Our results showed that the recombinant alphaviral plasmid vaccine pSCA1-E2-Erns-Cap-Rep was successfully constructed. The vaccine encoding E2 and Erns of CSFV, Cap, and Rep of PCV2 can induce E2, Erns, Cap, and Rep protein expression. ELISA analysis showed that mice-immunized pSCA1-E2-Erns-Cap-Rep plasmid vaccine produced higher anti–CSFV- and anti–PCV2-specific antibodies with dose- and time-dependent manners. Furthermore, neutralizing assays were measured using IF and ELISA methods. The results showed the production of neutralizing antibodies could neutralize CSFV (up to 210.13) and PCV2 (28.6) effectively, which exhibited the immune efficacy of the pSCA1-E2-Erns-Cap-Rep plasmid vaccine. Taken together, this pSCA1-E2-Erns-Cp-Rep plasmid vaccine could be considered a novel candidate vaccine against CSFV and PCV2. |
| first_indexed | 2024-04-11T13:37:43Z |
| format | Article |
| id | doaj.art-7a0e06003a7e4b37a543183234682576 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T13:37:43Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7a0e06003a7e4b37a5431832346825762022-12-22T04:21:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10655321065532Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep proteinFuyu Du0Fuyu Du1Zhi Cao2Zixuan Ye3Jun He4Weijie Zhang5Ke Zhang6Pengbo Ning7Pengbo Ning8Pengbo Ning9School of Life Science and Technology, Xidian University, Xi’an, ChinaShaoxing Academy of Biomedicine of Zhejiang Sci-Tech University, Shaoxing, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaSchool of Life Science and Technology, Xidian University, Xi’an, ChinaSchool of Life Science and Technology, Xidian University, Xi’an, ChinaSchool of Life Science and Technology, Xidian University, Xi’an, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaSchool of Life Science and Technology, Xidian University, Xi’an, ChinaShaoxing Academy of Biomedicine of Zhejiang Sci-Tech University, Shaoxing, ChinaEngineering Research Center of Molecular and Neuro Imaging Ministry of Education, School of Life Science and Technology, Xidian University, Xi’an, ChinaClassical swine fever virus (CSFV) and porcine Circovirus type 2 (PCV2) are economically pivotal infectious disease viruses of swine. Alphaviral RNA replicon plasmids have been used as an important vector for constructing nucleic acid vaccines. Here, we aimed to construct a recombinant alphaviral plasmid vaccine pSCA1-E2-Erns-Cap-Rep for the prevention and control of CSFV and PCV2. Our results showed that the recombinant alphaviral plasmid vaccine pSCA1-E2-Erns-Cap-Rep was successfully constructed. The vaccine encoding E2 and Erns of CSFV, Cap, and Rep of PCV2 can induce E2, Erns, Cap, and Rep protein expression. ELISA analysis showed that mice-immunized pSCA1-E2-Erns-Cap-Rep plasmid vaccine produced higher anti–CSFV- and anti–PCV2-specific antibodies with dose- and time-dependent manners. Furthermore, neutralizing assays were measured using IF and ELISA methods. The results showed the production of neutralizing antibodies could neutralize CSFV (up to 210.13) and PCV2 (28.6) effectively, which exhibited the immune efficacy of the pSCA1-E2-Erns-Cap-Rep plasmid vaccine. Taken together, this pSCA1-E2-Erns-Cp-Rep plasmid vaccine could be considered a novel candidate vaccine against CSFV and PCV2.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1065532/fullDNA bivalent vaccineCSFVPCV2coinfectionalphaviral plasmid |
| spellingShingle | Fuyu Du Fuyu Du Zhi Cao Zixuan Ye Jun He Weijie Zhang Ke Zhang Pengbo Ning Pengbo Ning Pengbo Ning Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein Frontiers in Microbiology DNA bivalent vaccine CSFV PCV2 coinfection alphaviral plasmid |
| title | Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein |
| title_full | Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein |
| title_fullStr | Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein |
| title_full_unstemmed | Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein |
| title_short | Production and immunogenicity of a deoxyribonucleic acid Alphavirus vaccine expressing classical swine fever virus E2-Erns protein and porcine Circovirus Cap-Rep protein |
| title_sort | production and immunogenicity of a deoxyribonucleic acid alphavirus vaccine expressing classical swine fever virus e2 erns protein and porcine circovirus cap rep protein |
| topic | DNA bivalent vaccine CSFV PCV2 coinfection alphaviral plasmid |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1065532/full |
| work_keys_str_mv | AT fuyudu productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT fuyudu productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT zhicao productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT zixuanye productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT junhe productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT weijiezhang productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT kezhang productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT pengboning productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT pengboning productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein AT pengboning productionandimmunogenicityofadeoxyribonucleicacidalphavirusvaccineexpressingclassicalswinefeverviruse2ernsproteinandporcinecircoviruscaprepprotein |