Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE
Fringes are glycosyltransferases that transfer a GlcNAc to <i>O</i>-fucose residues on Epidermal Growth Factor-like (EGF) repeats. Three Fringes exist in mammals: LUNATIC FRINGE (LFNG), MANIC FRINGE (MFNG), and RADICAL FRINGE (RFNG). Fringe modification of <i>O</i>-fucose on...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/19/5942 |
| _version_ | 1797515925371486208 |
|---|---|
| author | Florian Pennarubia Alison V. Nairn Megumi Takeuchi Kelley W. Moremen Robert S. Haltiwanger |
| author_facet | Florian Pennarubia Alison V. Nairn Megumi Takeuchi Kelley W. Moremen Robert S. Haltiwanger |
| author_sort | Florian Pennarubia |
| collection | DOAJ |
| description | Fringes are glycosyltransferases that transfer a GlcNAc to <i>O</i>-fucose residues on Epidermal Growth Factor-like (EGF) repeats. Three Fringes exist in mammals: LUNATIC FRINGE (LFNG), MANIC FRINGE (MFNG), and RADICAL FRINGE (RFNG). Fringe modification of <i>O</i>-fucose on EGF repeats in the NOTCH1 (N1) extracellular domain modulates the activation of N1 signaling. Not all <i>O</i>-fucose residues of N1 are modified by all Fringes; some are modified by one or two Fringes and others not modified at all. The distinct effects on N1 activity depend on which Fringe is expressed in a cell. However, little data is available on the effect that more than one Fringe has on the modification of <i>O</i>-fucose residues and the resulting downstream consequence on Notch activation. Using mass spectral glycoproteomic site mapping and cell-based N1 signaling assays, we compared the effect of co-expression of N1 with one or more Fringes on modification of <i>O</i>-fucose and activation of N1 in three cell lines. Individual expression of each Fringe with N1 in the three cell lines revealed differences in modulation of the Notch pathway dependent on the presence of endogenous Fringes. Despite these cell-based differences, co-expression of several Fringes with N1 demonstrated a dominant effect of LFNG over MFNG or RFNG. MFNG and RFNG appeared to be co-dominant but strongly dependent on the ligands used to activate N1 and on the endogenous expression of Fringes. These results show a hierarchy of Fringe activity and indicate that the effect of MFNG and/or RFNG could be small in the presence of LFNG. |
| first_indexed | 2024-03-10T06:54:06Z |
| format | Article |
| id | doaj.art-7a4749df810f4d3ca393d87ec8c7957d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:54:06Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7a4749df810f4d3ca393d87ec8c7957d2023-11-22T16:34:57ZengMDPI AGMolecules1420-30492021-09-012619594210.3390/molecules26195942Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGEFlorian Pennarubia0Alison V. Nairn1Megumi Takeuchi2Kelley W. Moremen3Robert S. Haltiwanger4Complex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USAComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USAComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USAComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USAComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USAFringes are glycosyltransferases that transfer a GlcNAc to <i>O</i>-fucose residues on Epidermal Growth Factor-like (EGF) repeats. Three Fringes exist in mammals: LUNATIC FRINGE (LFNG), MANIC FRINGE (MFNG), and RADICAL FRINGE (RFNG). Fringe modification of <i>O</i>-fucose on EGF repeats in the NOTCH1 (N1) extracellular domain modulates the activation of N1 signaling. Not all <i>O</i>-fucose residues of N1 are modified by all Fringes; some are modified by one or two Fringes and others not modified at all. The distinct effects on N1 activity depend on which Fringe is expressed in a cell. However, little data is available on the effect that more than one Fringe has on the modification of <i>O</i>-fucose residues and the resulting downstream consequence on Notch activation. Using mass spectral glycoproteomic site mapping and cell-based N1 signaling assays, we compared the effect of co-expression of N1 with one or more Fringes on modification of <i>O</i>-fucose and activation of N1 in three cell lines. Individual expression of each Fringe with N1 in the three cell lines revealed differences in modulation of the Notch pathway dependent on the presence of endogenous Fringes. Despite these cell-based differences, co-expression of several Fringes with N1 demonstrated a dominant effect of LFNG over MFNG or RFNG. MFNG and RFNG appeared to be co-dominant but strongly dependent on the ligands used to activate N1 and on the endogenous expression of Fringes. These results show a hierarchy of Fringe activity and indicate that the effect of MFNG and/or RFNG could be small in the presence of LFNG.https://www.mdpi.com/1420-3049/26/19/5942NOTCH1FringeEGF-like domains<i>O</i>-fucosylationglycosylation |
| spellingShingle | Florian Pennarubia Alison V. Nairn Megumi Takeuchi Kelley W. Moremen Robert S. Haltiwanger Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE Molecules NOTCH1 Fringe EGF-like domains <i>O</i>-fucosylation glycosylation |
| title | Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE |
| title_full | Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE |
| title_fullStr | Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE |
| title_full_unstemmed | Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE |
| title_short | Modulation of the NOTCH1 Pathway by LUNATIC FRINGE Is Dominant over That of MANIC or RADICAL FRINGE |
| title_sort | modulation of the notch1 pathway by lunatic fringe is dominant over that of manic or radical fringe |
| topic | NOTCH1 Fringe EGF-like domains <i>O</i>-fucosylation glycosylation |
| url | https://www.mdpi.com/1420-3049/26/19/5942 |
| work_keys_str_mv | AT florianpennarubia modulationofthenotch1pathwaybylunaticfringeisdominantoverthatofmanicorradicalfringe AT alisonvnairn modulationofthenotch1pathwaybylunaticfringeisdominantoverthatofmanicorradicalfringe AT megumitakeuchi modulationofthenotch1pathwaybylunaticfringeisdominantoverthatofmanicorradicalfringe AT kelleywmoremen modulationofthenotch1pathwaybylunaticfringeisdominantoverthatofmanicorradicalfringe AT robertshaltiwanger modulationofthenotch1pathwaybylunaticfringeisdominantoverthatofmanicorradicalfringe |