Production of Antimicrobial Defensin in Nicotiana benthamiana with a Potato Virus X Vector

Production of Antimicrobial Defensin in Nicotiana benthamiana with a Potato Virus X Vector

A recombinant plasmid, pTXS.TH, was constructed to express the gene-encoding wasabi (Wasabia japonica) defensin with the potato virus X (PVX) vector. pTXS.TH allows the expression of defensin in the host Nicotiana benthamiana, and the defensin protein WT1 can be purified from virus-infected leaves b...

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Bibliographic Details
Main Authors: Hiromasa Saitoh, Akinori Kiba, Masahiro Nishihara, Saburo Yamamura, Kazumi Suzuki, Ryohei Terauchi
Format: Article
Language:English
Published: The American Phytopathological Society 2001-02-01
Series:Molecular Plant-Microbe Interactions
Subjects:
degenerate primer
His6
immunoblot analysis
PCR
SDS-PAGE
Online Access:https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.2.111

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https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.2.111

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