A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

Summary: CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.org/kits/moclo-ytk/). This step-by-step pr...

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Bibliographic Details
Main Authors: Daniele Novarina, Andriana Koutsoumpa, Andreas Milias-Argeitis
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Genetics
Model Organisms
Molecular Biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002386

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722002386

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