A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

Summary: CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.org/kits/moclo-ytk/). This step-by-step pr...

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Bibliographic Details
Main Authors: Daniele Novarina, Andriana Koutsoumpa, Andreas Milias-Argeitis
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Genetics
Model Organisms
Molecular Biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002386
Description
Summary:Summary: CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.org/kits/moclo-ytk/). This step-by-step protocol guides the reader from sgRNA design to verification of the desired genome editing event and provides preassembled plasmids for cloning the sgRNA(s), making this technology easily accessible to any yeast research group.For complete details on the use and execution of this protocol, please refer to Novarina et al. (2021). : Publisher's note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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