Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning

Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning

Genetic engineering of complex metabolic pathways and multiple traits often requires the introduction of multiple genes. The construction of plasmids carrying multiple DNA fragments plays a vital role in these processes. In this study, the Gibson assembly and Gateway cloning combined Pyramiding Stac...

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Bibliographic Details
Main Authors: Dongdong Zeng, Cuiyuan Jing, Lin Tang, Peng He, Jie Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-11-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
multigene stacking
Gibson assembly
gateway cloning
pyramiding stacking of multigenes
genetic engineering
synthetic biology
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1263715/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2023.1263715/full

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