An improved CRISPRi system in Pichia pastoris

An improved CRISPRi system in Pichia pastoris

CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest...

Full description

Bibliographic Details
Main Authors: Shujing Qiao, Fan Bai, Peng Cai, Yongjin J. Zhou, Lun Yao
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2023-09-01
Series:Synthetic and Systems Biotechnology
Subjects:
Pichia pastoris
CRISPRi
HH/HDV
Csy4
gRNA array
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X23000534

Internet

http://www.sciencedirect.com/science/article/pii/S2405805X23000534

Similar Items