Does filter-aided sample preparation provide sufficient method linearity for quantitative plant shotgun proteomics?

Does filter-aided sample preparation provide sufficient method linearity for quantitative plant shotgun proteomics?

Due to its outstanding throughput and analytical resolution, gel-free LC-based shotgun proteomics represents the gold standard of proteome analysis. Thereby, the efficiency of sample preparation dramatically affects the correctness and reliability of protein quantification. Thus, the steps of protei...

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Bibliographic Details
Main Authors: Tatiana Leonova, Christian Ihling, Mohamad Saoud, Nadezhda Frolova, Robert Rennert, Ludger A. Wessjohann, Andrej Frolov
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Plant Science
Subjects:
detergent-assisted proteolysis
filter aided sample preparation (FASP)
label-free quantification
LC-MS
phenol extraction
plant proteomics
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.874761/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2022.874761/full

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