Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways
Neuroinflammation is recognized as an important pathogenic factor for aging and related cognitive disorders. Mitogen-activated protein kinase and nuclear factor kappa B signaling pathways may mediate neuroinflammation. Saponins from Panax japonicus are the most abundant and bioactive members in rhiz...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=11;spage=1877;epage=1884;aulast=Deng |
| _version_ | 1818213985905803264 |
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| author | Li-li Deng Ding Yuan Zhi-yong Zhou Jing-zhi Wan Chang-cheng Zhang Chao-qi Liu Yao-yan Dun Hai-xia Zhao Bo Zhao Yuan-jian Yang Ting Wang |
| author_facet | Li-li Deng Ding Yuan Zhi-yong Zhou Jing-zhi Wan Chang-cheng Zhang Chao-qi Liu Yao-yan Dun Hai-xia Zhao Bo Zhao Yuan-jian Yang Ting Wang |
| author_sort | Li-li Deng |
| collection | DOAJ |
| description | Neuroinflammation is recognized as an important pathogenic factor for aging and related cognitive disorders. Mitogen-activated protein kinase and nuclear factor kappa B signaling pathways may mediate neuroinflammation. Saponins from Panax japonicus are the most abundant and bioactive members in rhizomes of Panax japonicus, and show anti-inflammatory activity. However, it is not known whether saponin from Panax japonicus has an anti-inflammatory effect in the aging brain, and likewise its underlying mechanisms. Sprague-Dawley rats were divided into control groups (3-, 9-, 15-, and 24-month-old groups) and saponins from Panax japonicus-treated groups. Saponins from Panax japonicus-treated groups were orally administrated saponins from Panax japonicus at three doses of 10, 30, and 60 mg/kg once daily for 6 months until the rats were 24 months old. Immunohistochemical staining and western blot assay results demonstrated that many microglia were activated in 24-month-old rats compared with 3- and 9-month-old rats. Expression of interleukin-1β, tumor necrosis factor-α, cyclooxygenase-2, and inducible nitric oxide synthase increased. Each dose of saponins from Panax japonicus visibly suppressed microglial activation in the aging rat brain, and inhibited expression levels of the above factors. Each dose of saponins from Panax japonicus markedly diminished levels of nuclear factor kappa B, IκBα, extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38. These results confirm that saponins from Panax japonicus can mitigate neuroinflammation in the aging rat brain by inhibition of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways. |
| first_indexed | 2024-12-12T06:13:00Z |
| format | Article |
| id | doaj.art-7ad9608eaf5849a6b7eef62be66507a8 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-12T06:13:00Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-7ad9608eaf5849a6b7eef62be66507a82022-12-22T00:35:06ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742017-01-0112111877188410.4103/1673-5374.219047Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathwaysLi-li DengDing YuanZhi-yong ZhouJing-zhi WanChang-cheng ZhangChao-qi LiuYao-yan DunHai-xia ZhaoBo ZhaoYuan-jian YangTing WangNeuroinflammation is recognized as an important pathogenic factor for aging and related cognitive disorders. Mitogen-activated protein kinase and nuclear factor kappa B signaling pathways may mediate neuroinflammation. Saponins from Panax japonicus are the most abundant and bioactive members in rhizomes of Panax japonicus, and show anti-inflammatory activity. However, it is not known whether saponin from Panax japonicus has an anti-inflammatory effect in the aging brain, and likewise its underlying mechanisms. Sprague-Dawley rats were divided into control groups (3-, 9-, 15-, and 24-month-old groups) and saponins from Panax japonicus-treated groups. Saponins from Panax japonicus-treated groups were orally administrated saponins from Panax japonicus at three doses of 10, 30, and 60 mg/kg once daily for 6 months until the rats were 24 months old. Immunohistochemical staining and western blot assay results demonstrated that many microglia were activated in 24-month-old rats compared with 3- and 9-month-old rats. Expression of interleukin-1β, tumor necrosis factor-α, cyclooxygenase-2, and inducible nitric oxide synthase increased. Each dose of saponins from Panax japonicus visibly suppressed microglial activation in the aging rat brain, and inhibited expression levels of the above factors. Each dose of saponins from Panax japonicus markedly diminished levels of nuclear factor kappa B, IκBα, extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38. These results confirm that saponins from Panax japonicus can mitigate neuroinflammation in the aging rat brain by inhibition of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways.http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=11;spage=1877;epage=1884;aulast=Dengnerve regeneration; saponins from Panax japonicus; aging; neuroinflammation; microglia; inflammatory factor; mitogen-activated protein kinase; nuclear factor kappa B; extracellular signal-regulated kinase; c-Jun N-terminal kinase; p38; neural regeneration |
| spellingShingle | Li-li Deng Ding Yuan Zhi-yong Zhou Jing-zhi Wan Chang-cheng Zhang Chao-qi Liu Yao-yan Dun Hai-xia Zhao Bo Zhao Yuan-jian Yang Ting Wang Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways Neural Regeneration Research nerve regeneration; saponins from Panax japonicus; aging; neuroinflammation; microglia; inflammatory factor; mitogen-activated protein kinase; nuclear factor kappa B; extracellular signal-regulated kinase; c-Jun N-terminal kinase; p38; neural regeneration |
| title | Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways |
| title_full | Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways |
| title_fullStr | Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways |
| title_full_unstemmed | Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways |
| title_short | Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways |
| title_sort | saponins from panax japonicus attenuate age related neuroinflammation via regulation of the mitogen activated protein kinase and nuclear factor kappa b signaling pathways |
| topic | nerve regeneration; saponins from Panax japonicus; aging; neuroinflammation; microglia; inflammatory factor; mitogen-activated protein kinase; nuclear factor kappa B; extracellular signal-regulated kinase; c-Jun N-terminal kinase; p38; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=11;spage=1877;epage=1884;aulast=Deng |
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