CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coli

CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coli

Abstract Background One major mission of microbial breeding is high-level production of desired metabolites. Overproduction of intermediate metabolites in core pathways is challenging as it may impair cell growth and viability. Results Here we report that aconitic acid, an intermediate metabolite in...

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Bibliographic Details
Main Authors: Qingyang Li, Peng Zhao, Hang Yin, Zhaonan Liu, Haifeng Zhao, Pingfang Tian
Format: Article
Language:English
Published: BMC 2020-09-01
Series:Microbial Cell Factories
Subjects:
Aconitic acid
CRISPR interference
Glucose metabolism
Isocitrate dehydrogenase
Pyruvate kinase
Online Access:http://link.springer.com/article/10.1186/s12934-020-01435-9

Internet

http://link.springer.com/article/10.1186/s12934-020-01435-9

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