Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris

Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris

Abstract Pichia pastoris (Komagataella phaffii) is widely used for industrial production of heterologous proteins due to high secretory capabilities but selection of highly productive engineered strains remains a limiting step. Despite availability of a comprehensive molecular toolbox for construct...

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Bibliographic Details
Main Authors: Laura Navone, Kaylee Moffitt, James Behrendorff, Pawel Sadowski, Carol Hartley, Robert Speight
Format: Article
Language:English
Published: BMC 2023-05-01
Series:Microbial Cell Factories
Subjects:
Protein production
Screening
Biosensor
Split GFP
Yeast
Online Access:https://doi.org/10.1186/s12934-023-02089-z

Internet

https://doi.org/10.1186/s12934-023-02089-z

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