Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization
Abstract Background Andrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production o...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-09-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-02946-2 |
| _version_ | 1827724267138056192 |
|---|---|
| author | Tingfeng Cheng Tongling Ge Lunqiang Zhao Yuyong Hou Jianye Xia Lei Zhao |
| author_facet | Tingfeng Cheng Tongling Ge Lunqiang Zhao Yuyong Hou Jianye Xia Lei Zhao |
| author_sort | Tingfeng Cheng |
| collection | DOAJ |
| description | Abstract Background Andrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production of andrimid to overcome the excessive costs of chemosynthesis. In screening for species with broad-spectrum antibacterial activity, we detected andrimid in the fermentation products of Erwinia persicina BST187. To increase andrimid production, the BST187 fermentation medium formulation and fermentation conditions were optimized by using systematic design of experiments (One-Factor-At-A-Time, Plackett–Burman design, Response Surface Methodology). Results The results indicate that the actual andrimid production reached 140.3 ± 1.28 mg/L under the optimized conditions (trisodium citrate dihydrate-30 g/L, beef extract-17.1 g/L, MgCl2·6H2O-100 mM, inoculation amount-1%, initial pH-7.0, fermentation time-36 h, temperature-19.7℃), which is 20-fold greater than the initial condition without optimization (7.00 ± 0.40 mg/L), consistent with the improved antibacterial effect of the fermentation supernatant. Conclusions The present study provides valuable information for improving andrimid production via optimization of the fermentation process, which will be of great value in the future industrialization of andrimid production. |
| first_indexed | 2024-03-10T22:12:25Z |
| format | Article |
| id | doaj.art-7b2c71ea9cb448b5b048ee5741b3ed7b |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-10T22:12:25Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-7b2c71ea9cb448b5b048ee5741b3ed7b2023-11-19T12:32:59ZengBMCBMC Microbiology1471-21802023-09-0123111410.1186/s12866-023-02946-2Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimizationTingfeng Cheng0Tongling Ge1Lunqiang Zhao2Yuyong Hou3Jianye Xia4Lei Zhao5Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesAbstract Background Andrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production of andrimid to overcome the excessive costs of chemosynthesis. In screening for species with broad-spectrum antibacterial activity, we detected andrimid in the fermentation products of Erwinia persicina BST187. To increase andrimid production, the BST187 fermentation medium formulation and fermentation conditions were optimized by using systematic design of experiments (One-Factor-At-A-Time, Plackett–Burman design, Response Surface Methodology). Results The results indicate that the actual andrimid production reached 140.3 ± 1.28 mg/L under the optimized conditions (trisodium citrate dihydrate-30 g/L, beef extract-17.1 g/L, MgCl2·6H2O-100 mM, inoculation amount-1%, initial pH-7.0, fermentation time-36 h, temperature-19.7℃), which is 20-fold greater than the initial condition without optimization (7.00 ± 0.40 mg/L), consistent with the improved antibacterial effect of the fermentation supernatant. Conclusions The present study provides valuable information for improving andrimid production via optimization of the fermentation process, which will be of great value in the future industrialization of andrimid production.https://doi.org/10.1186/s12866-023-02946-2AndrimidErwinia persicinaAntibacterial activityFermentationDesign of experimentResponse surface methodology |
| spellingShingle | Tingfeng Cheng Tongling Ge Lunqiang Zhao Yuyong Hou Jianye Xia Lei Zhao Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization BMC Microbiology Andrimid Erwinia persicina Antibacterial activity Fermentation Design of experiment Response surface methodology |
| title | Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization |
| title_full | Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization |
| title_fullStr | Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization |
| title_full_unstemmed | Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization |
| title_short | Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization |
| title_sort | improved production of andrimid in erwinia persicina bst187 strain by fermentation optimization |
| topic | Andrimid Erwinia persicina Antibacterial activity Fermentation Design of experiment Response surface methodology |
| url | https://doi.org/10.1186/s12866-023-02946-2 |
| work_keys_str_mv | AT tingfengcheng improvedproductionofandrimidinerwiniapersicinabst187strainbyfermentationoptimization AT tonglingge improvedproductionofandrimidinerwiniapersicinabst187strainbyfermentationoptimization AT lunqiangzhao improvedproductionofandrimidinerwiniapersicinabst187strainbyfermentationoptimization AT yuyonghou improvedproductionofandrimidinerwiniapersicinabst187strainbyfermentationoptimization AT jianyexia improvedproductionofandrimidinerwiniapersicinabst187strainbyfermentationoptimization AT leizhao improvedproductionofandrimidinerwiniapersicinabst187strainbyfermentationoptimization |