Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells
Exposure to organic dust is a risk factor for the development of respiratory diseases. Surfactant proteins (SP) reduce alveolar surface tension and modulate innate immune responses to control lung inflammation. Therefore, changes in SP levels could contribute to the development of organic-dust-induc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2019-02-01
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| Series: | Innate Immunity |
| Online Access: | https://doi.org/10.1177/1753425919827360 |
| _version_ | 1828268844133646336 |
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| author | Kartiga Natarajan Keerthi Gangam Velmurugan Meganathan Koteswara R Gottipati Courtney Mitchell Vijay Boggaram |
| author_facet | Kartiga Natarajan Keerthi Gangam Velmurugan Meganathan Koteswara R Gottipati Courtney Mitchell Vijay Boggaram |
| author_sort | Kartiga Natarajan |
| collection | DOAJ |
| description | Exposure to organic dust is a risk factor for the development of respiratory diseases. Surfactant proteins (SP) reduce alveolar surface tension and modulate innate immune responses to control lung inflammation. Therefore, changes in SP levels could contribute to the development of organic-dust-induced respiratory diseases. Because information on the effects of organic dust on SP levels is lacking, we studied the effects of dust from a poultry farm on SP expression. We found that dust extract reduced SP-A and SP-B mRNA and protein levels in H441 human lung epithelial cells by inhibiting their promoter activities, but did not have any effect on SP-D protein levels. Dust extract also reduced SP-A and SP-C levels in primary human alveolar epithelial cells. The inhibitory effects were not due to LPS or protease activities present in dust extract or mediated via oxidative stress, but were dependent on a heat-labile factor(s). Thyroid transcription factor-1, a key transcriptional activator of SP expression, was reduced in dust-extract-treated cells, indicating that its down-regulation mediates inhibition of SP levels. Our study implies that down-regulation of SP levels by organic dust could contribute to the development of lung inflammation and respiratory diseases in humans. |
| first_indexed | 2024-04-13T05:24:02Z |
| format | Article |
| id | doaj.art-7b4b2a19d4f84981901bf5160fb01660 |
| institution | Directory Open Access Journal |
| issn | 1753-4259 1753-4267 |
| language | English |
| last_indexed | 2024-04-13T05:24:02Z |
| publishDate | 2019-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Innate Immunity |
| spelling | doaj.art-7b4b2a19d4f84981901bf5160fb016602022-12-22T03:00:39ZengSAGE PublishingInnate Immunity1753-42591753-42672019-02-012510.1177/1753425919827360Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cellsKartiga NatarajanKeerthi GangamVelmurugan MeganathanKoteswara R GottipatiCourtney MitchellVijay BoggaramExposure to organic dust is a risk factor for the development of respiratory diseases. Surfactant proteins (SP) reduce alveolar surface tension and modulate innate immune responses to control lung inflammation. Therefore, changes in SP levels could contribute to the development of organic-dust-induced respiratory diseases. Because information on the effects of organic dust on SP levels is lacking, we studied the effects of dust from a poultry farm on SP expression. We found that dust extract reduced SP-A and SP-B mRNA and protein levels in H441 human lung epithelial cells by inhibiting their promoter activities, but did not have any effect on SP-D protein levels. Dust extract also reduced SP-A and SP-C levels in primary human alveolar epithelial cells. The inhibitory effects were not due to LPS or protease activities present in dust extract or mediated via oxidative stress, but were dependent on a heat-labile factor(s). Thyroid transcription factor-1, a key transcriptional activator of SP expression, was reduced in dust-extract-treated cells, indicating that its down-regulation mediates inhibition of SP levels. Our study implies that down-regulation of SP levels by organic dust could contribute to the development of lung inflammation and respiratory diseases in humans.https://doi.org/10.1177/1753425919827360 |
| spellingShingle | Kartiga Natarajan Keerthi Gangam Velmurugan Meganathan Koteswara R Gottipati Courtney Mitchell Vijay Boggaram Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells Innate Immunity |
| title | Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells |
| title_full | Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells |
| title_fullStr | Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells |
| title_full_unstemmed | Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells |
| title_short | Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells |
| title_sort | organic dust inhibits surfactant protein expression by reducing thyroid transcription factor 1 levels in human lung epithelial cells |
| url | https://doi.org/10.1177/1753425919827360 |
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