Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model
<p>Abstract</p> <p>Background</p> <p>Human T cell leukemia virus type I (HTLV-I) causes adult T-cell leukemia (ATL) in infected individuals after a long incubation period. Immunological studies have suggested that insufficient host T cell response to HTLV-I is a potenti...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-10-01
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| Series: | Retrovirology |
| Online Access: | http://www.retrovirology.com/content/5/1/90 |
| _version_ | 1811329340052340736 |
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| author | Takayanagi Ryo Okada Hiroyuki Nagai Mika Ohashi Takashi Shida Hisatoshi |
| author_facet | Takayanagi Ryo Okada Hiroyuki Nagai Mika Ohashi Takashi Shida Hisatoshi |
| author_sort | Takayanagi Ryo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Human T cell leukemia virus type I (HTLV-I) causes adult T-cell leukemia (ATL) in infected individuals after a long incubation period. Immunological studies have suggested that insufficient host T cell response to HTLV-I is a potential risk factor for ATL. To understand the relationship between host T cell response and HTLV-I pathogenesis in a rat model system, we have developed an activation and detection system of HTLV-I Tax-specific cytotoxic T lymphocytes (CTLs) by Epitope expressing Single-Chain Trimers (SCTs) of MHC Class I.</p> <p>Results</p> <p>We have established expression vectors which encode SCTs of rat MHC-I (RT1.A<sup>l</sup>) with Tax180-188 peptide. Human cell lines transfected with the established expression vectors were able to induce IFN-γ and TNF-α production by a Tax180-188-specific CTL line, 4O1/C8. We have further fused the C-terminus of SCTs to EGFP and established cells expressing SCT-EGFP fusion protein on the surface. By co-cultivating the cells with 4O1/C8, we have confirmed that the epitope-specific CTLs acquired SCT-EGFP fusion proteins and that these EGFP-possessed CTLs were detectable by flow cytometric analysis.</p> <p>Conclusion</p> <p>We have generated a SCT of rat MHC-I linked to Tax epitope peptide, which can be applicable for the induction of Tax-specific CTLs in rat model systems of HTLV-I infection. We have also established a detection system of Tax-specific CTLs by using cells expressing SCTs fused with EGFP. These systems will be useful tools in understanding the role of HTLV-I specific CTLs in HTLV-I pathogenesis.</p> |
| first_indexed | 2024-04-13T15:42:15Z |
| format | Article |
| id | doaj.art-7b4ed3c63f9349cc8d6119517a2132f1 |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-04-13T15:42:15Z |
| publishDate | 2008-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-7b4ed3c63f9349cc8d6119517a2132f12022-12-22T02:41:06ZengBMCRetrovirology1742-46902008-10-01519010.1186/1742-4690-5-90Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat modelTakayanagi RyoOkada HiroyukiNagai MikaOhashi TakashiShida Hisatoshi<p>Abstract</p> <p>Background</p> <p>Human T cell leukemia virus type I (HTLV-I) causes adult T-cell leukemia (ATL) in infected individuals after a long incubation period. Immunological studies have suggested that insufficient host T cell response to HTLV-I is a potential risk factor for ATL. To understand the relationship between host T cell response and HTLV-I pathogenesis in a rat model system, we have developed an activation and detection system of HTLV-I Tax-specific cytotoxic T lymphocytes (CTLs) by Epitope expressing Single-Chain Trimers (SCTs) of MHC Class I.</p> <p>Results</p> <p>We have established expression vectors which encode SCTs of rat MHC-I (RT1.A<sup>l</sup>) with Tax180-188 peptide. Human cell lines transfected with the established expression vectors were able to induce IFN-γ and TNF-α production by a Tax180-188-specific CTL line, 4O1/C8. We have further fused the C-terminus of SCTs to EGFP and established cells expressing SCT-EGFP fusion protein on the surface. By co-cultivating the cells with 4O1/C8, we have confirmed that the epitope-specific CTLs acquired SCT-EGFP fusion proteins and that these EGFP-possessed CTLs were detectable by flow cytometric analysis.</p> <p>Conclusion</p> <p>We have generated a SCT of rat MHC-I linked to Tax epitope peptide, which can be applicable for the induction of Tax-specific CTLs in rat model systems of HTLV-I infection. We have also established a detection system of Tax-specific CTLs by using cells expressing SCTs fused with EGFP. These systems will be useful tools in understanding the role of HTLV-I specific CTLs in HTLV-I pathogenesis.</p>http://www.retrovirology.com/content/5/1/90 |
| spellingShingle | Takayanagi Ryo Okada Hiroyuki Nagai Mika Ohashi Takashi Shida Hisatoshi Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model Retrovirology |
| title | Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model |
| title_full | Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model |
| title_fullStr | Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model |
| title_full_unstemmed | Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model |
| title_short | Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model |
| title_sort | activation and detection of htlv i tax specific ctls by epitope expressing single chain trimers of mhc class i in a rat model |
| url | http://www.retrovirology.com/content/5/1/90 |
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