Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation
Abstract Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry. Results Mitochondria isolated from murine quadriceps femori...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-09-01
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Series: | BMC Research Notes |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13104-023-06519-4 |