Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

Abstract Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry. Results Mitochondria isolated from murine quadriceps femori...

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Bibliographic Details
Main Authors: Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza
Format: Article
Language:English
Published: BMC 2023-09-01
Series:BMC Research Notes
Subjects:
Online Access:https://doi.org/10.1186/s13104-023-06519-4