Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

Abstract Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry. Results Mitochondria isolated from murine quadriceps femori...

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Bibliographic Details
Main Authors: Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza
Format: Article
Language:English
Published: BMC 2023-09-01
Series:BMC Research Notes
Subjects:
Percoll purification
Density gradient
Mitochondria
Bioenergetics
Skeletal muscle
Online Access:https://doi.org/10.1186/s13104-023-06519-4

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