A quantitative approach to detect and overcome PCR inhibition in ancient DNA extracts

A quantitative approach to detect and overcome PCR inhibition in ancient DNA extracts

Inhibition is problematic in many applications of PCR, particularly those involving degraded or low amounts of template DNA, when simply diluting the extract is undesirable. Two basic approaches to monitoring inhibition in such samples using real-time or quantitative PCR (qPCR) have been proposed. T...

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Bibliographic Details
Main Authors: Christine E. King, Régis Debruyne, Melanie Kuch, Carsten Schwarz, Hendrik N. Poinar
Format: Article
Language:English
Published: Taylor & Francis Group 2009-11-01
Series:BioTechniques
Subjects:
PCR inhibitors
quantitative PCR
amplification efficiency
degraded DNA
forensic DNA analysis
PCR kinetics
Online Access:https://www.future-science.com/doi/10.2144/000113244

Internet

https://www.future-science.com/doi/10.2144/000113244

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