The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma
Abstract Background Allergic asthma is a type I allergic reaction mediated by serum Immunoglobulin E (IgE). B cell-mediated humoral immune response to allergens in the pathophysiology of allergic asthma have not been thoroughly elucidated. Peripheral helper T cells (Tph) and follicular helper T cell...
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| פורמט: | Article |
| שפה: | English |
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BMC
2024-02-01
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| סדרה: | BMC Pediatrics |
| נושאים: | |
| גישה מקוונת: | https://doi.org/10.1186/s12887-024-04622-4 |
| _version_ | 1827326182492733440 |
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| author | Yunying Zhu Qian Jiang Chenshuang Lei Qinhua Yu Liannv Qiu |
| author_facet | Yunying Zhu Qian Jiang Chenshuang Lei Qinhua Yu Liannv Qiu |
| author_sort | Yunying Zhu |
| collection | DOAJ |
| description | Abstract Background Allergic asthma is a type I allergic reaction mediated by serum Immunoglobulin E (IgE). B cell-mediated humoral immune response to allergens in the pathophysiology of allergic asthma have not been thoroughly elucidated. Peripheral helper T cells (Tph) and follicular helper T cells (Tfh) promote B cell differentiation and antibody production in inflamed tissues. Objective To investigate the roles of B cell subsets, Tph cell subsets and Tfh cell subsets in allergic immune responses. Methods Circulating B cell subsets, Tph cell subsets and Tfh cell subsets in 33 children with allergic asthma and 17 healthy children were analyzed using multicolor flow cytometry. The level of serum total IgE was also assessed. Results Our study found that CD27+CD38+ plasmablasts and CD24hiCD38hi transitional B cells increased and were correlated with serum total IgE level, CD27− naive B cells and CD24hiCD27+ B cells decreased in children with allergic asthma. CXCR5− Tph, CXCR5−ICOS+ Tph, CXCR5−ICOS+PD-1+ Tph, CXCR5+ICOS+ Tfh and CXCR5+ICOS+PD-1+ Tfh increased in children with allergic asthma. Further analysis showed increased Tph2, Tph17, Tfh2 and Tfh17 subtypes while decreased Tph1 and Tfh1 subtypes in children with allergic asthma. Most interestingly, Tph2 or Tfh2 subtypes had a positive correlation with serum total IgE level. Conclusion Overall, these results provide insight into the allergens elicited B, Tph or Tfh cell response and identify heretofore unappreciated CD24hiCD38hi transitional B cells, CD24hiCD27+ B cells, CXCR5− Tph, CXCR5−ICOS+PD-1+ Tph, Tph2 subtypes and Tfh2 subtypes response to allergens. |
| first_indexed | 2024-03-07T14:40:51Z |
| format | Article |
| id | doaj.art-7b6b56763a874028a16e2abbcb625dc6 |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-03-07T14:40:51Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-7b6b56763a874028a16e2abbcb625dc62024-03-05T20:23:01ZengBMCBMC Pediatrics1471-24312024-02-012411810.1186/s12887-024-04622-4The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthmaYunying Zhu0Qian Jiang1Chenshuang Lei2Qinhua Yu3Liannv Qiu4Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People’s Hospital(Affiliated People’s Hospital),Hangzhou Medical CollegeDepartment of Clinical Laboratory, Ningbo Puji Hospital (Ningbo Second Hospital West Hospital)Department of Clinical Laboratory, Wenzhou Central HospitalLaboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People’s Hospital(Affiliated People’s Hospital),Hangzhou Medical CollegeLaboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People’s Hospital(Affiliated People’s Hospital),Hangzhou Medical CollegeAbstract Background Allergic asthma is a type I allergic reaction mediated by serum Immunoglobulin E (IgE). B cell-mediated humoral immune response to allergens in the pathophysiology of allergic asthma have not been thoroughly elucidated. Peripheral helper T cells (Tph) and follicular helper T cells (Tfh) promote B cell differentiation and antibody production in inflamed tissues. Objective To investigate the roles of B cell subsets, Tph cell subsets and Tfh cell subsets in allergic immune responses. Methods Circulating B cell subsets, Tph cell subsets and Tfh cell subsets in 33 children with allergic asthma and 17 healthy children were analyzed using multicolor flow cytometry. The level of serum total IgE was also assessed. Results Our study found that CD27+CD38+ plasmablasts and CD24hiCD38hi transitional B cells increased and were correlated with serum total IgE level, CD27− naive B cells and CD24hiCD27+ B cells decreased in children with allergic asthma. CXCR5− Tph, CXCR5−ICOS+ Tph, CXCR5−ICOS+PD-1+ Tph, CXCR5+ICOS+ Tfh and CXCR5+ICOS+PD-1+ Tfh increased in children with allergic asthma. Further analysis showed increased Tph2, Tph17, Tfh2 and Tfh17 subtypes while decreased Tph1 and Tfh1 subtypes in children with allergic asthma. Most interestingly, Tph2 or Tfh2 subtypes had a positive correlation with serum total IgE level. Conclusion Overall, these results provide insight into the allergens elicited B, Tph or Tfh cell response and identify heretofore unappreciated CD24hiCD38hi transitional B cells, CD24hiCD27+ B cells, CXCR5− Tph, CXCR5−ICOS+PD-1+ Tph, Tph2 subtypes and Tfh2 subtypes response to allergens.https://doi.org/10.1186/s12887-024-04622-4Allergic asthmaFlow cytometryB cell subsetsPeripheral helper T cellIgE |
| spellingShingle | Yunying Zhu Qian Jiang Chenshuang Lei Qinhua Yu Liannv Qiu The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma BMC Pediatrics Allergic asthma Flow cytometry B cell subsets Peripheral helper T cell IgE |
| title | The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma |
| title_full | The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma |
| title_fullStr | The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma |
| title_full_unstemmed | The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma |
| title_short | The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma |
| title_sort | response of cd27 cd38 plasmablasts cd24hicd38hi transitional b cells cxcr5 icos pd 1 tph tph2 and tfh2 subtypes to allergens in children with allergic asthma |
| topic | Allergic asthma Flow cytometry B cell subsets Peripheral helper T cell IgE |
| url | https://doi.org/10.1186/s12887-024-04622-4 |
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