Nile Red Detection of Bacterial Hydrocarbons and Ketones in a High-Throughput Format

ABSTRACT A method for use in high-throughput screening of bacteria for the production of long-chain hydrocarbons and ketones by monitoring fluorescent light emission in the presence of Nile red is described. Nile red has previously been used to screen for polyhydroxybutyrate (PHB) and fatty acid est...

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Bibliographic Details
Main Authors: Neissa M. Pinzon, Kelly G. Aukema, Jeffrey A. Gralnick, Lawrence P. Wackett
Format: Article
Language:English
Published: American Society for Microbiology 2011-09-01
Series:mBio
Online Access:https://journals.asm.org/doi/10.1128/mBio.00109-11