DNA extraction from clotted blood in genotyping quality

DNA extraction from clotted blood in genotyping quality

DNA extraction from frozen blood clots is challenging. Here, the authors applied QIAGEN Clotspin Baskets and the Gentra Puregene Blood Kit for DNA extraction to cellular fraction of 5.5 ml whole blood without anticoagulating additives. The amount and quality of extracted DNA were assessed via spectr...

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Bibliographic Details
Main Authors: Kira J Stanzick, Josef Simon, Martina E Zimmermann, Michael Schachtner, David Peterhoff, Hans-Helmut Niller, Klaus Überla, Ralf Wagner, Iris M Heid, Klaus J Stark
Format: Article
Language:English
Published: Taylor & Francis Group 2023-01-01
Series:BioTechniques
Subjects:
anticoagulated blood
AugUR
clotted blood
DNA concentration
DNA extraction
DNA purity
Online Access:https://www.future-science.com/doi/10.2144/btn-2022-0061

Internet

https://www.future-science.com/doi/10.2144/btn-2022-0061

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