Triptolide attenuates irritable bowel syndrome via inhibiting ODC1
Abstract Background Irritable bowel syndrome (IBS) is a chronic disorder of the gut-brain axis with significant morbidity. Triptolide, an active compound extracted from Tripterygium wilfordii Hook F (TwHF), has been widely used as a major medicinal herb in the treatment of inflammatory disease. Meth...
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| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-023-02847-8 |
| _version_ | 1797801378379202560 |
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| author | Ning Zhu Liuyan Zhu Xueliang Zhang Chengbin Huang Wenjun Xiang Bingwu Huang |
| author_facet | Ning Zhu Liuyan Zhu Xueliang Zhang Chengbin Huang Wenjun Xiang Bingwu Huang |
| author_sort | Ning Zhu |
| collection | DOAJ |
| description | Abstract Background Irritable bowel syndrome (IBS) is a chronic disorder of the gut-brain axis with significant morbidity. Triptolide, an active compound extracted from Tripterygium wilfordii Hook F (TwHF), has been widely used as a major medicinal herb in the treatment of inflammatory disease. Methods The chronic-acute combined stress (CAS) stimulation was used to establish IBS rat model. The model rats were then gavaged with triptolide. Forced swimming, marble-burying, fecal weight and abdominal withdrawal reflex (AWR) score were recorded. Pathologic changes in the ileal and colonic tissues were validated by hematoxylin and eosin staining. The inflammatory cytokines and Ornithine Decarboxylase-1 (ODC1) in the ileal and colonic tissues were performed by ELISA and WB. Results Triptolide didn’t have antidepressant- and antianxiety- effects in rats caused by CAS, but decreased fecal weight and AWR score. In addition, Triptolide reduced the release of IL-1, IL-6, and TNF-α and the expression of ODC1 in the ileum and colon. Conclusion The therapeutic efficacy of triptolide for IBS induced by CAS was revealed in this study, which may be related to the reduction of ODC1. |
| first_indexed | 2024-03-13T04:49:34Z |
| format | Article |
| id | doaj.art-7b92a88b05b64457ae78cf261081dc0e |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-03-13T04:49:34Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-7b92a88b05b64457ae78cf261081dc0e2023-06-18T11:16:04ZengBMCBMC Gastroenterology1471-230X2023-06-012311810.1186/s12876-023-02847-8Triptolide attenuates irritable bowel syndrome via inhibiting ODC1Ning Zhu0Liuyan Zhu1Xueliang Zhang2Chengbin Huang3Wenjun Xiang4Bingwu Huang5Department of Cardiology, The Third Affiliated Hospital of Shanghai University (Wenzhou People’s Hospital), The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical UniversityDepartment of General Practice, The Third Affiliated Hospital of Shanghai University (Wenzhou People’s Hospital), The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical UniversityDepartment of General Practice, The Third Affiliated Hospital of Shanghai University (Wenzhou People’s Hospital), The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical UniversityDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Yuying Children’s Hospital of Wenzhou Medical UniversityDepartment of Pathology, The Third Affiliated Hospital of Shanghai University (Wenzhou People’s Hospital), The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical UniversityDepartment of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital, Yuying Children’s Hospital of Wenzhou Medical UniversityAbstract Background Irritable bowel syndrome (IBS) is a chronic disorder of the gut-brain axis with significant morbidity. Triptolide, an active compound extracted from Tripterygium wilfordii Hook F (TwHF), has been widely used as a major medicinal herb in the treatment of inflammatory disease. Methods The chronic-acute combined stress (CAS) stimulation was used to establish IBS rat model. The model rats were then gavaged with triptolide. Forced swimming, marble-burying, fecal weight and abdominal withdrawal reflex (AWR) score were recorded. Pathologic changes in the ileal and colonic tissues were validated by hematoxylin and eosin staining. The inflammatory cytokines and Ornithine Decarboxylase-1 (ODC1) in the ileal and colonic tissues were performed by ELISA and WB. Results Triptolide didn’t have antidepressant- and antianxiety- effects in rats caused by CAS, but decreased fecal weight and AWR score. In addition, Triptolide reduced the release of IL-1, IL-6, and TNF-α and the expression of ODC1 in the ileum and colon. Conclusion The therapeutic efficacy of triptolide for IBS induced by CAS was revealed in this study, which may be related to the reduction of ODC1.https://doi.org/10.1186/s12876-023-02847-8TriptolideIrritable bowel syndromeInflammationOrnithine decarboxylase 1 |
| spellingShingle | Ning Zhu Liuyan Zhu Xueliang Zhang Chengbin Huang Wenjun Xiang Bingwu Huang Triptolide attenuates irritable bowel syndrome via inhibiting ODC1 BMC Gastroenterology Triptolide Irritable bowel syndrome Inflammation Ornithine decarboxylase 1 |
| title | Triptolide attenuates irritable bowel syndrome via inhibiting ODC1 |
| title_full | Triptolide attenuates irritable bowel syndrome via inhibiting ODC1 |
| title_fullStr | Triptolide attenuates irritable bowel syndrome via inhibiting ODC1 |
| title_full_unstemmed | Triptolide attenuates irritable bowel syndrome via inhibiting ODC1 |
| title_short | Triptolide attenuates irritable bowel syndrome via inhibiting ODC1 |
| title_sort | triptolide attenuates irritable bowel syndrome via inhibiting odc1 |
| topic | Triptolide Irritable bowel syndrome Inflammation Ornithine decarboxylase 1 |
| url | https://doi.org/10.1186/s12876-023-02847-8 |
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