Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells
Capparis spinosa L. (C. spinosa) has been used as food and traditional medicine and shows anti-inflammatory and anti-oxidant activities. Here, we prepared the C. spinosa fruit ethanol extracts (CSEs) using different procedures and investigated the effects of CSE on the maturation of mouse bone marro...
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| Format: | Article |
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MDPI AG
2017-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/1/97 |
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| author | Azeguli Hamuti Jinyu Li Fangfang Zhou Adila Aipire Ji Ma Jianhua Yang Jinyao Li |
| author_facet | Azeguli Hamuti Jinyu Li Fangfang Zhou Adila Aipire Ji Ma Jianhua Yang Jinyao Li |
| author_sort | Azeguli Hamuti |
| collection | DOAJ |
| description | Capparis spinosa L. (C. spinosa) has been used as food and traditional medicine and shows anti-inflammatory and anti-oxidant activities. Here, we prepared the C. spinosa fruit ethanol extracts (CSEs) using different procedures and investigated the effects of CSE on the maturation of mouse bone marrow-derived dendritic cells (DCs) in the absence or presence of lipopolysaccharide (LPS). DC maturation and cytokine production were detected by flow cytometry and ELISA, respectively. We obtained three different CSEs and dissolved in water or DMSO, named CSE2W, CSEMW, CSE3W, CSE2D, CSEMD, and CSE3D, respectively. These CSEs showed different effects on DC maturation. CSEMW and CSEMD significantly increased the expressions of CD40, CD80, and CD86, in a dose-dependent manner. CSE2W and CSE2D also showed a modest effect on DC maturation, which enhanced the expression of CD40. CSE3W and CSE3D did not change DC maturation but suppressed LPS-induced DC maturation characterized by the decreased levels of CD40 and CD80. CSE3W and CSE3D also significantly inhibited the secretions of IL-12p40, IL-6, IL-1β, and TNF-α induced by LPS. CSE3W further increased the level of IL-10 induced by LPS. Moreover, CSE3D suppressed LPS-induced DC maturation in vivo, which decreased the expressions of CD40 and CD80. These results suggested that CSE3W and CSE3D might be used to treat inflammatory diseases. |
| first_indexed | 2024-12-13T09:48:36Z |
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| id | doaj.art-7b9c07785afa424cba52499896485605 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T09:48:36Z |
| publishDate | 2017-01-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7b9c07785afa424cba524998964856052022-12-21T23:51:59ZengMDPI AGMolecules1420-30492017-01-012219710.3390/molecules22010097molecules22010097Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic CellsAzeguli Hamuti0Jinyu Li1Fangfang Zhou2Adila Aipire3Ji Ma4Jianhua Yang5Jinyao Li6Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi 830046, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 666 Shengli Road, Urumqi 830046, ChinaCapparis spinosa L. (C. spinosa) has been used as food and traditional medicine and shows anti-inflammatory and anti-oxidant activities. Here, we prepared the C. spinosa fruit ethanol extracts (CSEs) using different procedures and investigated the effects of CSE on the maturation of mouse bone marrow-derived dendritic cells (DCs) in the absence or presence of lipopolysaccharide (LPS). DC maturation and cytokine production were detected by flow cytometry and ELISA, respectively. We obtained three different CSEs and dissolved in water or DMSO, named CSE2W, CSEMW, CSE3W, CSE2D, CSEMD, and CSE3D, respectively. These CSEs showed different effects on DC maturation. CSEMW and CSEMD significantly increased the expressions of CD40, CD80, and CD86, in a dose-dependent manner. CSE2W and CSE2D also showed a modest effect on DC maturation, which enhanced the expression of CD40. CSE3W and CSE3D did not change DC maturation but suppressed LPS-induced DC maturation characterized by the decreased levels of CD40 and CD80. CSE3W and CSE3D also significantly inhibited the secretions of IL-12p40, IL-6, IL-1β, and TNF-α induced by LPS. CSE3W further increased the level of IL-10 induced by LPS. Moreover, CSE3D suppressed LPS-induced DC maturation in vivo, which decreased the expressions of CD40 and CD80. These results suggested that CSE3W and CSE3D might be used to treat inflammatory diseases.http://www.mdpi.com/1420-3049/22/1/97Capparis spinosadendritic cellmaturationcytokine production |
| spellingShingle | Azeguli Hamuti Jinyu Li Fangfang Zhou Adila Aipire Ji Ma Jianhua Yang Jinyao Li Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells Molecules Capparis spinosa dendritic cell maturation cytokine production |
| title | Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells |
| title_full | Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells |
| title_fullStr | Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells |
| title_full_unstemmed | Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells |
| title_short | Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells |
| title_sort | capparis spinosa fruit ethanol extracts exert different effects on the maturation of dendritic cells |
| topic | Capparis spinosa dendritic cell maturation cytokine production |
| url | http://www.mdpi.com/1420-3049/22/1/97 |
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